Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence.
Analysis of the Cellular Stress Response During Ebola Virus Infection by Immunofluorescence.
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通过免疫荧光分析埃博拉病毒感染期间的细胞应激反应。
DOI：
10.1007/978-1-4939-7116-9_17
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Schmidt,KristinaM
中科院分区：
文献类型：
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作者：
Nelson,EmilyV;Schmidt,KristinaM
在本章中，描述了使用免疫荧光分析作为检查埃博拉病毒（EBOV）感染细胞中应激颗粒（SG）形成的工具。下面的方案侧重于诱导和分析细胞应激反应的过程，包括用SG形成的诱导剂和抑制剂处理细胞，还描述了EBOV感染，DNA转染和不同细胞系的使用。
In this chapter, the use of immunofluorescence analysis as a tool to examine stress granule (SG) formation in Ebola virus (EBOV)-infected cells is described. The following protocol focuses on the process of inducing and analyzing the cellular stress response, including treatment of cells with inducers and inhibitors of the SG formation, and also describes EBOV infection, DNA transfection, and the usage of different cell lines.