Increasing pCO2 correlates with low concentrations of intracellular dimethylsulfoniopropionate in the sea anemone Anemonia viridis.
Increasing pCO2 correlates with low concentrations of intracellular dimethylsulfoniopropionate in the sea anemone Anemonia viridis.
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DOI：
10.1002/ece3.946
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发表时间：
2014-02
影响因子：
2.6
通讯作者：
Fine, Maoz
中科院分区：
文献类型：
--
作者：
Borell, Esther M.;Steinke, Michael;Horwitz, Rael;Fine, Maoz
海洋珊瑚虫与鞭毛藻保持共生关系，鞭毛藻是藻类次级代谢物二甲基磺丙酸酯（DMSP）的多产生产者，DMSP是气候冷却微量气体二甲基硫化物（DMS）的前体。令人惊讶的是，关于DMSP在珊瑚虫中的生理作用和调节其产生的环境因素知之甚少。在这里，我们评估了DMSP作为抗氧化剂的潜在功能作用，并确定了未来海水pCO2的增加如何影响意大利Vulcano岛天然pCO2梯度的海葵Anemonia viridis中的DMSP浓度。在梯度V1 （3232 μatm CO2）、V2 （682 μatm）和对照（463 μatm） 3个站点的海葵之间，虫黄藻基因型、特征（虫黄藻密度、叶绿素a）和蛋白质浓度均无显著差异，说明绿藻能够适应不同的海水pCO2。相比之下，V1站海葵（33.23±8.30 fmol cell−1）和V2站海葵（34.78±8.69 fmol cell−1）的DMSP浓度比对照站海葵触角（51.85±12.96 fmol cell−1）的DMSP浓度低35%左右。此外，DMSP的低组织浓度与抗氧化酶超氧化物歧化酶（SOD）的低活性相一致。V1处理下宿主（7.84±1.37 U·mg−1蛋白）和虫黄藻（2.84±0.41 U·mg−1蛋白）的超氧化物歧化酶活性均比对照站（宿主：13.19±1.42；虫黄藻：4.72±0.57 U·mg−1蛋白）低40%。我们的研究结果为预测环境变化下沿海DMSP的产生提供了新的思路，并支持DMSP在共生珊瑚虫中作为抗氧化剂的功能。
Marine anthozoans maintain a mutualistic symbiosis with dinoflagellates that are prolific producers of the algal secondary metabolite dimethylsulfoniopropionate (DMSP), the precursor of the climate-cooling trace gas dimethyl sulfide (DMS). Surprisingly, little is known about the physiological role of DMSP in anthozoans and the environmental factors that regulate its production. Here, we assessed the potential functional role of DMSP as an antioxidant and determined how future increases in seawater pCO2 may affect DMSP concentrations in the anemone Anemonia viridis along a natural pCO2 gradient at the island of Vulcano, Italy. There was no significant difference in zooxanthellae genotype and characteristics (density of zooxanthellae, and chlorophyll a) as well as protein concentrations between anemones from three stations along the gradient, V1 (3232 μatm CO2), V2 (682 μatm) and control (463 μatm), which indicated that A. viridis can acclimate to various seawater pCO2. In contrast, DMSP concentrations in anemones from stations V1 (33.23 ± 8.30 fmol cell−1) and V2 (34.78 ± 8.69 fmol cell−1) were about 35% lower than concentrations in tentacles from the control station (51.85 ± 12.96 fmol cell−1). Furthermore, low tissue concentrations of DMSP coincided with low activities of the antioxidant enzyme superoxide dismutase (SOD). Superoxide dismutase activity for both host (7.84 ± 1.37 U·mg−1 protein) and zooxanthellae (2.84 ± 0.41 U·mg−1 protein) at V1 was 40% lower than at the control station (host: 13.19 ± 1.42; zooxanthellae: 4.72 ± 0.57 U·mg−1 protein). Our results provide insight into coastal DMSP production under predicted environmental change and support the function of DMSP as an antioxidant in symbiotic anthozoans.
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影响因子：
1.6
作者：
DYKENS, JA;SHICK, JM
通讯作者：
SHICK, JM
DOI：
10.1073/pnas.0907163107
发表时间：
2010-01-12
影响因子：
11.1
作者：
Hopkins, Frances E.;Turner, Suzanne M.;Liss, Peter S.
通讯作者：
Liss, Peter S.
影响因子：
64.8
作者：
BATES, TS;CHARLSON, RJ;GAMMON, RH
通讯作者：
GAMMON, RH
影响因子：
5.8
作者：
Boatta, F.;D'Alessandro, W.;Parello, F.
通讯作者：
Parello, F.
影响因子：
4.3
作者：
Avgoustidi, Valia;Nightingale, Philip D.;Liss, Peter S.
通讯作者：
Liss, Peter S.