The HLA-A*0201-restricted H-Y antigen contains a posttranslationally modified cysteine that significantly affects T cell recognition
The HLA-A*0201-restricted H-Y antigen contains a posttranslationally modified cysteine that significantly affects T cell recognition
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DOI：
10.1016/s1074-7613(00)80330-1
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发表时间：
1997-03-01
期刊：
影响因子：
32.4
通讯作者：
Engelhard, VH
中科院分区：
文献类型：
--
作者：
Meadows, L;Wang, W;Engelhard, VH
鉴定了一种由两种细胞毒性T细胞克隆识别的肽，所述细胞毒性T细胞克隆对人类次要组织相容性抗原H-Y具有特异性，并受HLA-A*0201限制。该肽来源于SMCY，其他两个H-Y表位也是如此，支持该蛋白作为小鼠和人类H-Y决定簇的主要来源的重要性。在天然加工的肽中，T细胞仅识别这种肽的后修饰形式，其在第七位经历了半胱氨酸残基的修饰。这些修饰之一涉及通过二硫键连接第二个半胱氨酸残基。这种修饰对T细胞识别有深远的影响，也发生在其他I类MHC相关肽中，支持其作为免疫决定簇的普遍重要性。
A peptide recognized by two cytotoxic T cell clones specific for the human minor histocompatibility antigen H-Y and restricted by HLA-A*0201 was identified. This peptide originates from SMCY, as do two other H-Y epitopes, supporting the importance of this protein as a major source of H-Y determinants in mice and humans. In naturally processed peptides, T cells only recognize posttranslationally altered forms of this peptide that have undergone modification of a cysteine residue in the seventh position. One of these modifications involves attachment of a second cysteine residue via a disulfide bond. This modification has profound effects on T cell recognition and also occurs In other class I MHC-associated peptides, supporting its general importance as an immunological determinant.