CD24 is expressed specifically in the nucleus pulposus of intervertebral discs
CD24 is expressed specifically in the nucleus pulposus of intervertebral discs
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DOI：
10.1016/j.bbrc.2005.10.166
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发表时间：
2005-12-30
影响因子：
3.1
通讯作者：
Suda, T
中科院分区：
文献类型：
--
作者：
Fujita, N;Miyamoto, T;Suda, T
椎间盘（IVD）由位于其中心的软凝胶状物质（髓核（NP））和周围的纤维软骨（纤维环（AF））组成。尽管IVD变性患者的数量，基因表达分析尚未进行NP，因此很少有人知道NP中表达的分子标志物。在这里，我们进行了一个微阵列屏幕在NP与其他九个组织，以确定特定的细胞表面标记NP。与其他组织相比，在10，490个基因中鉴定出5个膜相关分子为NP中高表达的基因。其中，我们确定了CD 24，糖基磷脂酰肌醇（GPI）锚蛋白作为NP的细胞表面标志物。CD24的表达也被检测到在突出的NP和脊索瘤，一种恶性肿瘤原发性脊索细胞来源，而软骨肉瘤是缺席。因此，CD24是NP和IVD疾病的分子标志物。(c)2005年爱思唯尔公司All rights reserved.
Intervertebral disc (IVD) consists of a soft gelatinous material in its center, the nucleus pulposus (NP), bounded peripherally by fibrocartilage, annulus fibrosus (AF). Despite the number of patients with IVD degeneration, gene expression analysis has not been undertaken in NP and therefore little is known about the molecular markers expressed in NP. Here, we undertook a microarray screen in NP with the other nine tissues to identify the specific cell surface markers for NP. Five membrane associating molecules out of 10,490 genes were identified as highly expressing genes in NP compared with the other tissues. Among them, we identified CD24, a glycosylphosphatidylinositol (GPI) anchor protein as a cell surface marker for NP. CD24 expression was also detected in the herniated NP and chordoma, a malignant primary tumor derived from notochordal cells, while it was absent in chondrosarcoma. Therefore, CD24 is a molecular marker for NP as well as the diseases of IVD. (c) 2005 Elsevier Inc. All rights reserved.