Micro-patterned surface construction on BCP ceramics and the regulation on inflammation-involved osteogenic differentiation
Micro-patterned surface construction on BCP ceramics and the regulation on inflammation-involved osteogenic differentiation
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BCP陶瓷的微图案表面构建及其对炎症相关成骨分化的调节
DOI：
10.1016/j.msec.2020.111220
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发表时间：
2020-11-01
影响因子：
7.9
通讯作者：
Li, Danyang
中科院分区：
文献类型：
--
作者：
Wang, Jing;Su, Yangyang;Li, Danyang
生物陶瓷的表面特性在调节成骨过程中的细胞事件方面起着至关重要的作用。然而，关于表面特性衍生的炎症对成骨的影响的了解尚不清楚。本研究制备了具有不同表面微图案的致密双相磷酸钙陶瓷，以研究表面形貌对巨噬细胞行为和功能的影响，以及随后对成骨前细胞成骨分化的级联调控。我们发现，在不同大小的微图案中，单个凸起的表面粗糙度和润湿性的变化会对巨噬细胞的黏附、分布和形态产生显著的影响。具体地说，单个凸面面积较小的图案化表面比平坦表面更有可能驱动巨噬细胞向M1样表型转变，这是由M1特异性标志物的基因表达和分子分泌上调决定的。此外，巨噬细胞/支架衍生液对MC3T3-E1细胞的成骨分化有明显的促进作用，提示BCP陶瓷的表面特性在支架诱导的炎症反应中起重要作用，这可能有助于其成骨潜能。
The surface characteristics of bioceramics are critical in modulating the cellular events in osteogenesis. However, understanding on the effect of surface properties-derived inflammation on osteogenesis is unclear. In this study, dense biphase calcium phosphate (BCP) ceramics with various surface micropatterns were fabricated to investigate the effect of surface morphologies on the behaviors and functions of macrophages, and the subsequent cascade regulation on the osteogenic differentiation of preosteoblast. We found that the change of surface roughness and wettability of single convex in micropatterns with different sizes could make dramatic differences on the adherence, distribution and morphology of macrophages. Specifically, the patterned surface with smaller single convex area was more likely to drive macrophages towards M1-like phenotype, as was determined by the upregulated gene expression and molecular secretion of M1-specific markers compared with flat surface. Moreover, the macrophages/scaffolds-derived medium showed an obvious promotion on osteogenic differentiation of MC3T3-E1 cells that suggested the surface characteristics of BCP ceramics are important in terms of scaffolds-induced inflammation, which might contribute to its osteogenesis potential.