FACTORS AFFECTING THE ORAL UPTAKE AND TRANSLOCATION OF POLYSTYRENE NANOPARTICLES - HISTOLOGICAL AND ANALYTICAL EVIDENCE
FACTORS AFFECTING THE ORAL UPTAKE AND TRANSLOCATION OF POLYSTYRENE NANOPARTICLES - HISTOLOGICAL AND ANALYTICAL EVIDENCE
复制标题
DOI：
10.3109/10611869509015936
复制
发表时间：
1995-01-01
影响因子：
4.5
通讯作者：
JANI, PU
中科院分区：
文献类型：
--
作者：
FLORENCE, AT;HILLERY, AM;JANI, PU
本文简要回顾了我们实验室通过组织学（定性）和分析测量聚苯乙烯水平（定量）对聚苯乙烯乳胶纳米颗粒吸收和移位的定量和定性证据。我们以前比较了非离子化的聚苯乙烯胶乳的吸收范围从50 nm到3 μ m的大小，并进行了一些羧基化微球和非离子系统之间的吸收比较，显示前者通过胃肠道淋巴组织的吸收较低。尺寸是一个关键参数，吸收随粒径减小而增加。早期的证据表明，摄取是通过派尔集合淋巴结和肠道相关淋巴组织（GALT）的其他元素。亲水性嵌段共聚物吸附到聚苯乙烯上显著降低肠道GALT的摄取。用特异性配体修饰表面，例如通过共价连接番茄凝集素分子，表明在它们与肠细胞结合并被肠细胞内化后，被非GALT组织广泛摄取。减少和增加摄取的能力是一种现象的明确证据，该现象具有进一步控制的潜力，以允许其完全用于药物或疫苗递送。迄今为止，纳米颗粒作为不稳定药物（例如蛋白质）的载体系统通过口服途径的证据仍有待证实。
Quantitative and qualitative evidence from our laboratories on the absorption and translocation of polystyrene latex nanoparticles both by histological (qualitative) and analytical measurement of levels of polystyrene (quantitative) is briefly reviewed in this paper. We have previously compared the uptake of nonionized polystyrene latex ranging in size from 50nm to 3 mu m, and made some comparisons of uptake between carboxylated microspheres and nonionic systems, showing the lower uptake of the former through the lymphoid tissue of the gastrointestinal tract. Size is a key parameter, uptake increasing with decreasing particle diameter. Early evidence suggested that uptake is by way of the Peyer's patches and other elements of the gut associated lymphoid tissue (GALT). Adsorption of hydrophilic block-copolymers onto polystyrene markedly reduces the uptake by intestinal GALT. Modification of the surface with specific ligands such as by covalent attachment of tomato lectin molecules has indicated widespread uptake by non-GALT tissues, following their binding to and internalisation by enterocytes. The ability to decrease and increase uptake is clear evidence of a phenomenon which has the potential for further control to allow it to be exploited fully for drug or vaccine delivery The evidence to date with nanoparticles as carriers systems for labile drugs such as proteins by the oral route remains to be substantiated.