Chloride salinity in a chloride-sensitive plant: Focusing on photosynthesis, hormone synthesis and transduction in tobacco.
Chloride salinity in a chloride-sensitive plant: Focusing on photosynthesis, hormone synthesis and transduction in tobacco.
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DOI：
10.1016/j.plaphy.2020.05.021
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发表时间：
2020-05
期刊：
影响因子：
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通讯作者：
Lin Wang;Jia-yang Xu;W. Jia;Zheng Chen;Zicheng Xu
中科院分区：
文献类型：
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作者：
Lin Wang;Jia-yang Xu;W. Jia;Zheng Chen;Zicheng Xu
氯(Cl−)是一种微量营养素，是植物生长的有益离子，但过量的Cl−容易导致Cl−盐度升高。烟草是对氯离子−敏感的物种，当遇到过量的氯离子−时，烟草会受到严重的伤害。然而，过量的Cl-−延缓植物生长发育的分子机制在很大程度上还不清楚。本研究测定了烟草植株暴露于12h、24h和48h的Cl-−盐度下的生理生化和遗传反应。与对照相比，Cl-−盐度增加了烟草叶片中Cl-−的含量，降低了叶片的相对含水量，严重限制了烟草的光合作用能力，减少了光合作用产物，降低了生长素和赤霉素的含量。此外，烟草叶片中的淀粉、总酚含量增加，苯丙氨酸解氨酶(PAL)活性增加，对Cl-−盐度的响应。多重组学结果表明，共有15,445个基因和1983个蛋白质在对Cl−盐度的响应中差异丰富。两条代谢途径，苯丙氨酸代谢和淀粉和蔗糖代谢，分别在转录组和蛋白质组学数据中被特异性地丰富。此外，我们对RNA-Seq和蛋白质组学数据的联合分析表明，734个差异丰富的基因/蛋白质主要在植物激素信号转导、光合作用和光合作用-天线蛋白途径中得到丰富。我们的工作不仅为烟草对氯离子−盐度的分子响应提供了新的见解，也为氯离子−敏感作物的改良提供了重要的指导。
Chloride (Cl−) is a micronutrient and a beneficial ion for plant growth, but excess Cl−easily leads to Cl−salinity. As a species sensitive to Cl−, tobacco experiences serious damage when encountering excessive Cl−. However, the molecular mechanism by which excess Cl−delays plant growth and development remain largely unknown. In this study, physiological, biochemical and genetic responses were determined in tobacco plants exposed to 12 h, 24 h and 48 h of Cl−salinity. Compared with the control, Cl−salinity increased the content of Cl−and decreased the relative water content (RWC) in tobacco, which severely limited the photosynthetic capacity and reduced photosynthetic products, resulting in decreased levels of auxin (IAA) and gibberellin (GA3). In addition, tobacco increased the content of starch, total phenol and increased phenylalanine ammonia-lyase (PAL) activity in response to Cl−salinity. Multi-omics results revealed that a total of 15,445 genes and 1983 proteins were differentially abundant in response to Cl−salinity. Two metabolic pathways, phenylalanine metabolism and starch and sucrose metabolism, were specifically enriched in the transcriptomic and proteomic data, respectively. In addition, our conjoint analysis of RNA-Seq and proteomics data revealed that 734 differentially abundant genes/proteins were enriched mainly in plant hormone signal transduction, photosynthesis and photosynthesis-antenna protein pathways. Our work presented here not only provides new insights into the molecular response of tobacco to Cl−salinity but also offers important guidance for the improvement of Cl−sensitive crops.