The plastid membranes of barley (Hordeum vulgare). Light-induced appearance of mRNA coding for the apoprotein of the light-harvesting chlorophyll a/b protein.
The plastid membranes of barley (Hordeum vulgare). Light-induced appearance of mRNA coding for the apoprotein of the light-harvesting chlorophyll a/b protein.
复制标题
大麦（Hordeum vulgare）的质体膜。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
K. Kloppstech
中科院分区：
文献类型：
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作者：
K. Apel;K. Kloppstech
黑暗生长的大麦植株的光照会诱导捕光叶绿素 a/b 蛋白大量插入正在发育的类囊体膜中。除了叶绿素合成的开始之外，光还特异性地诱导编码 Mr 29500 多肽的重要 mRNA 种类的出现。该成分被鉴定为光捕获叶绿素 a/b 蛋白的脱辅基蛋白的前体。该前体的 Mr 值比真实蛋白大大约 4000。对大麦无叶绿素 b 的突变体 lorina f2 的研究为控制光依赖性 mRNA 形成的机制提供了第一个线索。编码光捕获叶绿素 a/b 蛋白的前蛋白的 mRNA 的诱导似乎与光捕获结构的组装过程没有直接关系，并且不需要叶绿素 b。据认为，光通过与原叶绿素(ide)光转化为叶绿素(ide)不同的反应对mRNA形成发挥影响。
Illumination of dark-grown barley plants induces a massive insertion of the light-harvesting chlorophyll a/b protein into the developing thylakoid membrane. In addition to the onset of chlorophyll synthesis, light induces specifically the appearance of a prominent mRNA species which codes for a polypeptide of Mr 29500. This component was identified as a precursor of the apoprotein of the light-harvesting chlorophyll a/b protein. The precursor has an Mr larger than the authentic protein by approximately 4000. Studies of the chlorophyll-b-less mutant chlorina f2 of barley offer the first clue to the mechanism which controls the light-dependent mRNA formation. The induction of the mRNA coding for the aproprotein of the light-harvesting chlorophyll a/b protein does not seem to be linked directly to the assembly process of the light-harvesting structure and does not require chlorophyll b. It is proposed that light exerts its influence on the mRNA formation by a reaction which is different from the phototransformation of protochlorophyll(ide) to chlorophyll(ide).