Keratinocyte growth factor-2 accelerates wound healing in incisional wounds
Keratinocyte growth factor-2 accelerates wound healing in incisional wounds
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DOI：
10.1006/jsre.1998.5501
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发表时间：
1999-02-01
影响因子：
2.2
通讯作者：
Rampy, MA
中科院分区：
文献类型：
--
作者：
Jimenez, PA;Rampy, MA
背景角质形成细胞生长因子-2（KGF-2）又称成纤维细胞生长因子-10（FGF-10），是一种新发现的成纤维细胞生长因子家族成员。KGF-2与最近鉴定的大鼠FGF-10具有96%的相同性，并且特异性地刺激正常人表皮角质形成细胞的生长。进行本研究以检查局部施用的KGF-2在切口伤口愈合模型中的作用，KGF-2治疗导致切口伤口愈合的改善，其特征在于断裂强度、胶原蛋白含量和表皮厚度的芸香增加。将KGF-2局部应用于在Sprague-Dawley大鼠背部皮肤中制作的线性切口。使用Instron张力计进行生物力学测试，以确定断裂和拉伸强度。使用Sircol胶原测定法测定伤口胶原含量。采用Masson三色染色法测量创面表皮厚度。在创伤时单次局部施用KGF-2导致创伤后第5天的伤口断裂和拉伸强度增加。与缓冲液对照相比，KGF-2处理伤口的断裂强度显着更高（1 μ g，222.1 +/- 13.5 g，P = 0.0007; 4 μ g，248.7 +/- 15.4 g，P = 0.0001; 10 μ g，247.2 +/- 21.9 g，P = 0.001;缓冲液，141.0 +/- 9.7 g）。用KGF-2治疗后，创面表皮厚度和胶原含量明显增加。根据我们的研究结果，KGF-2是一种有效的伤口愈合刺激剂，表现为机械强度增加，伴随着伤口胶原蛋白含量的增加。KGF-2可能是一种重要的细胞介质，负责启动和加速伤口愈合，并可能促进外科伤口的愈合。(C)北京：科学出版社.
Background. Keratinocyte growth factor-2 (KGF-2) also described as fibroblast growth factor-10 (FGF-10) is a newly identified member of the fibroblast growth factor family. KGF-2 is 96% identical to the recently identified rat FGF-10 and specifically stimulates growth of normal human epidermal keratinocytes, The present study was undertaken to examine the effects of topically applied KGF-2 in an incisional wound healing model, KGF-2 treatment resulted in an improvement in incisional wound healing as characterized by rue increase in breaking strength, collagen content, and epidermal thickness.Methods. KGF-2 was topically applied to linear incisions made in the dorsal skin of Sprague-Dawley rats. Biomechanical testing was done using an Instron tensiometer for breaking and tensile strength determinations. Wound collagen content was determined using the Sircol collagen assay. Epidermal thickness measurements were conducted using Masson's trichromestained sections of the wound.Results. A single topical application of KGF-2 at the time of wounding resulted in an increase in wound breaking and tensile strength at Day 5 after wounding. Breaking strength of KGF-2-treated wounds was significantly higher compared with the buffer control (1 mu g, 222.1 +/- 13.5 g, P = 0.0007; 4 mu g, 248.7 +/- 15.4 g, P = 0.0001; 10 mu g, 247.2 +/- 21.9 g, P = 0.001; buffer, 141.0 +/- 9.7 g). Epiderm,al thickness and wound collagen content were significantly increased following treatment with KGF-2.Conclusions. Based on our findings, KGF-2 is a potent stimulator of wound healing as demonstrated by increased mechanical strength accompanied by an increase in wound collagen content. KGF-2 could be an important cellular mediator responsible for the initiation and acceleration of wound healing and may enhance the healing of surgical wounds. (C) 1999 Academic Press.