Ghrelin in neonatal rats: distribution in stomach and its possible role
Ghrelin in neonatal rats: distribution in stomach and its possible role
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DOI：
10.1677/joe.0.1730239
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发表时间：
2002-05-01
影响因子：
4
通讯作者：
Murakami, N
中科院分区：
文献类型：
--
作者：
Hayashida, T;Nakahara, K;Murakami, N
Ghrelin是一种由28个氨基酸组成的肽。最近从大鼠胃中分离出作为GH促分泌素受体的内源性配体。事实上，胃饥饿素，中枢或外周给药，刺激食物摄入和GH分泌表明，胃饥饿素在大鼠的生长中具有重要作用。我们用免疫组织化学和放射免疫测定法来确定生长激素释放肽免疫染色细胞开始出现在大鼠胃中的年龄。Ghrelin免疫反应细胞被发现表达在胎儿胃从怀孕第18天。生长素释放肽免疫反应细胞在胎儿胃的数量增加，随着胃的增长。在大鼠胃的腺体部分的胃促生长素的量也增加，以年龄依赖性的方式，从新生儿阶段到成人。8小时的牛奶限制显着降低了1周龄大鼠胃中的ghrelin浓度，并增加了血浆中的ghrelin浓度。给1周龄和3周龄大鼠施用ghrelin可增加血浆GH浓度。从妊娠第15天至第21天，每天皮下给予妊娠大鼠Ghrelin，导致新生大鼠体重增加。此外，在出生前每天皮下给予新生大鼠生长素释放肽，将阴道开放前30.7 +/-0.94天提前至27.9 +/-0.05天。这些结果表明，ghrelin可能参与新生儿的发育。
Ghrelin, a 28 amino acid peptide. has recently been isolated from the rat stomach as an endogenous ligand for the GH secretagogue receptor. The fact that administration of ghrelin, centrally or peripherally, stimulates both food intake and GH secretion suggests that stomach ghrelin has an important role in the growth of rats. We used immunohistochemistry and radioimmunoassay to determine the age at which ghrelin-immunostained cells begin to appear in the rat stomach. Ghrelin-immunoreactive cells were found to be expressed in the fetal stomach from pregnancy day 18. The number of ghrelin-immunoreactive cells in the fetal stomach increased as the stomach grew. The amount of ghrelin in the glandular part of the rat stomach also increased, in an age-dependent manner, from the neonatal stage to adult. Eight hours of milk restriction significantly decreased the ghrelin concentration in the stomachs of 1-week-old rats, and increased the ghrelin concentration in their plasma. Administration of ghrelin to 1- and 3-week-old rats increased plasma GH concentrations. The daily subcutaneous administration of ghrelin to pregnant rats from day 15 to day 21 of pregnancy caused in increase in body weight of newborn rats. In addition, daily subcutaneous administration of ghrelin to neonatal rats front birth advanced the day of vaginal opening front day 30.7 +/- 0.94 to day 27.9 +/- 0.05. These results suggest that ghrelin may be involved in neonatal development.