AN ALL-SOLID-STATE FLOW CYTOMETER FOR COUNTING FLUORESCENT MICROSPHERES
AN ALL-SOLID-STATE FLOW CYTOMETER FOR COUNTING FLUORESCENT MICROSPHERES
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DOI：
10.1021/ac00111a027
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发表时间：
1995-08-01
影响因子：
7.4
通讯作者：
WOLFRUM, J
中科院分区：
文献类型：
--
作者：
NIEHREN, S;KINZELBACH, W;WOLFRUM, J
颗粒示踪剂在裂隙介质研究中具有重要意义，由于其运移行为与溶质示踪剂不同，可提供有关裂隙空间的补充信息，这些信息在危险废物地下贮存场的风险评估中至关重要。到目前为止，这些颗粒的检测和计数是通过过滤样品的荧光显微镜进行的。为了允许更快和在线连续监测单个颗粒，在本工作中已经开发了流式细胞仪。该细胞仪由固态光学组件构建，从而产生适合现场使用的鲁棒和可运输的系统。为了最大化荧光量子效率，使用新染料JA 22，雪崩二极管的使用使得光子检测的效率最大化，对于完全的数字数据采集和分析，仅需要个人计算机，细胞仪的检测效率> 25%，探针现在为20 mL/h，并且目前的准确度为< 160个颗粒/mL。
Particle tracers are of interest in the investigation of fractured media, Because their transport behavior differs from that of solute tracers, they supply complementary information on fracture space, This information is crucial in the risk assessment of underground hazardous waste storage sites, An easy-to-handle particle tracer consists of fluorescent polystyrene spheres with diameters on the order of 1 mu m. Up to now, detection and counting of these particles was performed by fluorescence microscopy of filtered samples, To allow faster and on-line continuous monitoring of single particles, a flow cytometer has been developed in the present work, The cytometer is built from solid-state optical components, resulting in a robust and transportable system suitable for field use, To maximize the fluorescence quantum efficiency, a new dye, JA22, was used, Use of an avalanche diode allowed the efficiency of photon detection to be maximized, For complete digital data acquisition and analysis, only a personal computer is required, The detection efficiency of the cytometer is > 25%, the probe now is 20 mL/h, and the present accuracy is < 160 particles/mL.