Somatic and germ-line reverse mutation rates of the retrovirus-induced dilute coat-color mutation of DBA mice.
Somatic and germ-line reverse mutation rates of the retrovirus-induced dilute coat-color mutation of DBA mice.
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逆转录病毒诱导的 DBA 小鼠淡毛色突变的体细胞和种系回复突变率。
DOI：
10.1073/pnas.85.1.189
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发表时间：
1988
影响因子：
11.1
通讯作者：
Copeland,NG
中科院分区：
文献类型：
--
作者：
Seperack,PK;Strobel,MC;Corrow,DJ;Jenkins,NA;Copeland,NG
目前，DBA小鼠的稀释（dv）毛色突变提供了测量哺乳动物逆转录病毒诱导突变的相对体细胞和生殖系回复突变率的唯一简单方法。dv突变是由亲嗜性小鼠白血病病毒自发整合到稀释位点的非编码序列中产生的。dv突变的逆转通过前病毒切除发生，并通过涉及病毒长末端重复序列的同源重组事件介导。虽然许多独立的生殖系d+回复突变体已被确定，体细胞d+回复突变体还没有以前的报道。在过去的5年中，我们筛选了超过100万只dv突变纯合子小鼠，以确定我们是否可以识别体细胞d+回复突变体。这项调查导致体细胞D+回复突变体的鉴定，并提供了一个数据库，从中我们可以估计相对体细胞和生殖系切除频率的逆转录病毒在小鼠和推测的同源重组事件产生D+回复突变体等位基因的性质。
At present, the dilute (dv) coat-color mutation of DBA mice provides the only simple means for measuring the relative somatic and germ-line reverse mutation rates of retrovirus-induced mutations in mammals. The dv mutation was generated by the spontaneous integration of an ecotropic murine leukemia virus into noncoding sequences of the dilute locus. Reversion of the dv mutation occurs by provirus excision and is mediated by homologous recombination events involving the viral long terminal repeat sequences. Although numerous independent germ-line d+ revertants have been identified, somatic d+ revertants have not been reported previously. During the past 5 years, we have screened more than one million mice homozygous for the dv mutation to determine whether we could identify somatic d+ revertants. This survey has resulted in the identification of a somatic d+ revertant and has provided a data base from which we can estimate the relative somatic and germ-line excision frequencies of retroviruses in mice and speculate about the nature of homologous recombination events producing d+ revertant alleles.
染色体在发育中的作用
DOI：
--
发表时间：
1964
期刊：
影响因子：
--
作者：
M. Locke
通讯作者：
M. Locke