cdc2 and the regulation of mitosis: six interacting mcs genes.
cdc2 and the regulation of mitosis: six interacting mcs genes.
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DOI：
10.1016/0168-9525(89)90167-4
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发表时间：
1989-08
期刊：
影响因子：
3.3
通讯作者：
Lisa Molz;R. Booher;’. PaulYoung;David Beach
中科院分区：
文献类型：
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作者：
Lisa Molz;R. Booher;’. PaulYoung;David Beach
裂殖酵母cdc 2 - 3 w weel-50双突变体表现出与染色体分离的总体异常相关的温度敏感致死表型，并被称为有丝分裂灾难。为了鉴定可能与cdc 2蛋白激酶在有丝分裂调控中相互作用的新的遗传元件，我们分离了致死性双突变体的回复突变体。抑制突变定义了6个mcs基因（mcs：有丝分裂灾难抑制基因），它们与以下任何一个有丝分裂控制基因都不等位：cdc 2、wee 1、cdc 13、cdc 25、suc 1或nim 1。每个mcs突变相对于野生型在抑制有丝分裂灾难的能力上是隐性的。没有赋予一个致命的表型作为一个单一的突变体，但很少的突变体预计是无效的。mcs突变体和其他有丝分裂调控因子之间的遗传相互作用的多样性被揭示出来，包括以下例子。首先，mcs 2 cdc 2 w或mcs 6 cdc 2 w双突变体显示依赖于cdc 2的特定wee等位基因的细胞周期缺陷。第二，mcs 1 cdc 25 -22或mcs 4 cdc 25 -22双突变体都是无条件致死的，即使在cdc 25 -22正常允许的温度下。最后，功能丧失的wee 1突变对cdc 25表型的特征性抑制在mcs 3突变背景中被逆转。mcs基因定义了新的有丝分裂元件，这些元件可能是cdc 2蛋白激酶的激活剂或底物。
A cdc2-3w weel-50 double mutant of fission yeast displays a temperature-sensitive lethal phenotype that is associated with gross abnormalities of chromosome segregation and has been termed mitotic catastrophe. In order to identify new genetic elements that might interact with the cdc2 protein kinase in the regulation of mitosis, we have isolated revertants of the lethal double mutant. The suppressor mutations define six mcs genes (mcs: mitotic catastrophe suppressor) that are not allelic to any of the following mitotic control genes: cdc2, wee 1, cdc13, cdc25, suc1 or nim1. Each mcs mutation is recessive with respect to wild-type in its ability to suppress mitotic catastrophe. None confer a lethal phenotype as a single mutant but few of the mutants are expected to be nulls. A diverse range of genetic interactions between the mcs mutants and other mitotic regulators were uncovered, including the following examples. First, mcs2 cdc2w or mcs6 cdc2w double mutants display a cell cycle defect dependent on the specific wee allele of cdc2. Second, both mcs1 cdc25-22 or mcs4 cdc25-22 double mutants are nonconditionally lethal, even at a temperature normally permissive for cdc25-22. Finally, the characteristic suppression of the cdc25 phenotype by a loss-of-function wee1 mutation is reversed in a mcs3 mutant background. The mcs genes define new mitotic elements that might be activators or substrates of the cdc2 protein kinase.