Delayed nerve repair increases number of caspase 3 stained Schwann cells
Delayed nerve repair increases number of caspase 3 stained Schwann cells
复制标题
DOI：
10.1016/j.neulet.2009.03.075
复制
发表时间：
2009-05-29
影响因子：
2.5
通讯作者：
Dahlin, Lars B.
中科院分区：
文献类型：
--
作者：
Saito, Harukazu;Kanje, Martin;Dahlin, Lars B.
采用免疫组织化学方法检测大鼠坐骨神经损伤后即刻(0d)或延迟修复(30d、90d和180d)雪旺细胞中caspase-3的表达，以此作为雪旺细胞凋亡的指标。即刻修复后10d，损伤部位(SNL；中位[IQR]，15.2[7.0]%)和远端神经节段(SND；9.5[3.6]%)裂解caspase3染色的雪旺细胞显著增加。在延迟修复后，两个部位caspase染色的雪旺细胞也显著增加(SNL：22.0-27.1%；SND：18.5-22.1%；P<；0.05)。损伤侧背根神经节内可见caspase 3染色的裂解卫星细胞，但各时间点均未见染色的运动神经元或感觉神经元。延迟的神经修复与更明显的雪旺细胞凋亡相关，这可能是神经损伤和延迟修复后神经再生受损的原因。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
Caspase 3 staining in Schwann cells was investigated with immunohistochemistry, as a measure of Schwann cell apoptosis, after transection and immediate (day 0) or delayed rat sciatic nerve repair (30, 90 and 180 days post injury). Cleaved caspase 3 stained Schwann cells significantly increased at the site of lesion (SNL; median [IQR], 15.2 [7.0] %) and in the distal nerve segment (SND; 9.5 [3.6] %) 10 days after immediate repair. The number of cleaved caspase stained Schwann cells also increased significantly after delayed repair, irrespective of length of delay, at both locations (SNL: 22.0-27.1%; SND: 18.5-22.1%; p < 0.05). Some cleaved caspase 3 stained satellite cells were seen in dorsal root ganglia on the injured side, but no stained motor or sensory neurons were observed at any time-point. Delayed nerve repair is associated with more pronounced Schwann cell apoptosis which may explain impaired nerve regeneration after nerve injury and delayed repair. (C) 2009 Elsevier Ireland Ltd. All rights reserved.