Towards a new classification of Muscidae (Diptera): a comparison of hypotheses based on multiple molecular phylogenetic approaches
Towards a new classification of Muscidae (Diptera): a comparison of hypotheses based on multiple molecular phylogenetic approaches
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DOI：
10.1111/syen.12473
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发表时间：
2021-02-26
影响因子：
4.8
通讯作者：
Piwczynski, Marcin
中科院分区：
文献类型：
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作者：
Grzywacz, Andrzej;Trzeciak, Paulina;Piwczynski, Marcin
蝇科是一个种类繁多的双翅目动物，在形态和生活史上表现出非凡的多样性，无论是在幼年还是成年。蝇科的分类一直争论不休，大多数更高层次的关系仍然未知。在这项研究中，我们使用了多位点Sanger测序（mS-seq）、锚定杂交富集（AHE）和限制性位点相关DNA测序（RAD-seq）方法来研究蝇科内部的关系。在整体拓扑结构方面，AHE和RAD-seq的结果与mS-seq的结果基本一致，但系统基因组方法得到了更高的节点支持。所有分子方法的结果与主要基于成虫形态的传统分类相矛盾，但提供了重新解释未成熟阶段形态的机会。蝇科的分类重排如下：(1)Mesembrina Meigen和Polietes Rondani从Muscinae转移到Azeliinae；（ii） Reinwardtiinae stat. rev.作为与Azeliinae不同的亚科复活；（iii） Eginia Robineau-Desvoidy、Neohelina Malloch、Syngamoptera Schnabl和Xenotachina Malloch被转移到Reinwardtiinae stat. rev.。
Muscidae are a megadiverse dipteran family that exhibits extraordinary diversity in morphology and life history as both immatures and adults. The classification of Muscidae has been long debated, and most higher-level relationships remain unknown. In this study, we used multilocus Sanger sequencing (mS-seq), anchored hybrid enrichment (AHE) and restriction-site associated DNA sequencing (RAD-seq) approaches to examine relationships within Muscidae. The results from AHE and RAD-seq largely correspond to those obtained from mS-seq in terms of overall topology, yet phylogenomic approaches received much higher nodal support. The results from all molecular approaches contradict the traditional classification based predominantly on adult morphology, but provide an opportunity to re-interpret the morphology of immature stages. Rearrangements in Muscidae classification are proposed as follows: (i) Mesembrina Meigen and Polietes Rondani are transferred from Muscinae to Azeliinae; (ii) Reinwardtiinae stat. rev. is resurrected as a subfamily distinct from Azeliinae; (iii) Eginia Robineau-Desvoidy, Neohelina Malloch, Syngamoptera Schnabl and Xenotachina Malloch are transferred to Reinwardtiinae stat. rev.