Template‐Free Galvanic Nanostructuring of Gold Electrodes for Sensitive Electrochemical Biosensors
Template‐Free Galvanic Nanostructuring of Gold Electrodes for Sensitive Electrochemical Biosensors
复制标题
用于灵敏电化学生物传感器的金电极的无模板电纳米结构
DOI：
10.1002/elan.200904665
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发表时间：
2009
期刊：
影响因子：
3
通讯作者：
G. Flechsig
中科院分区：
文献类型：
--
作者：
F. Wachholz;H. Duwensee;Ralf Schmidt;M. Zwanzig;J. Gimsa;S. Fiedler;G. Flechsig
金纳米结构的光滑的金盘电极已进行电沉积。FE-SEM图像表明改性电极的电化学活性表面显著增大。循环伏安法中的金氧化物还原峰证实了这一点。该电极还可用于序列特异性DNA检测。为此，将互补的单链捕获探针固定在电极上，并与互补和非互补的[OsO 4（bipy）]标记的DNA靶点连续杂交。我们发现中间去杂交步骤不会降低对特异性靶的灵敏度。此外，表面改性增加了伏安信号约。因子9。此外，观察到增强的线性校准范围。所提出的简单的纳米结构化过程为许多电化学传感器应用提供了很大的希望。
Gold nanostructuring of smooth gold disk-electrodes has been performed by electrodeposition. FE-SEM images indicate a strongly enlarged electrochemically active surface of the modified electrodes. This could be approved by the gold oxide reduction peak in cyclic voltammetry. The electrodes were also applied for sequence-specific DNA detection. For this, complementary single stranded capture probes were immobilized on the electrodes and consecutively hybridized with complementary and noncomplementary [OsO4(bipy)]-labeled DNA targets. We found that intermediate dehybridization steps did not decrease the sensitivity for the specific target. Moreover, the surface modification increased the voltammetric signal by an approx. factor of 9. Further, an enhanced linear calibration range was observed. The presented simple nanostructuring process holds great promise for many electrochemical sensor applications.
影响因子：
14.9
作者：
Kelley, SO;Boon, EM;Hill, MG
通讯作者：
Hill, MG