Mesenchymal stromal cells improve the osteogenic capabilities of mineralized agarose gels in a rat full-thickness cranial defect model
Mesenchymal stromal cells improve the osteogenic capabilities of mineralized agarose gels in a rat full-thickness cranial defect model
复制标题
DOI：
10.1002/term.495
复制
发表时间：
2013-01-01
影响因子：
3.3
通讯作者：
Yura, Yoshiaki
中科院分区：
文献类型：
--
作者：
Mizuta, Norihiko;Hattori, Koji;Yura, Yoshiaki
作者之前创造了形成在琼脂糖凝胶(分别为HAP和CaCO3凝胶)上或其中的HAP或CaCO3作为生物相容和可生物降解的骨移植材料。然而，这些凝胶在骨再生方面有局限性。间充质基质细胞(MSCs)具有成骨潜能，被认为可用于骨组织工程。本研究的目的是利用大鼠颅骨缺损模型，比较骨髓间充质干细胞在HAP或CaCO3凝胶(分别为MSC/HAP和MSC/CaCO3凝胶)中的成骨能力。制备HAP、CaCO3、MSC/Hap和MSC/CaCO3凝胶用于体内分析，并将其植入大鼠颅骨造成的全层骨缺损中。所有标本在植入后4周和8周进行放射学和组织学检查。使用微焦点计算机断层扫描，观察到与单独使用凝胶相比，加载味精的凝胶中的骨形成增加。此外，外周定量计算机断层扫描显示，与单纯凝胶相比，负载MSC的凝胶中的骨矿物质含量更高。经X射线衍射分析，载MSC凝胶中新生骨的骨质量指标磷灰石c轴取向程度与活颅骨接近。组织学上，与单纯凝胶相比，在负载MSC的凝胶中可以检测到更广泛的骨形成。总体而言，MSC/HAP和MSC/CaCO3凝胶在骨再生方面显示出相同的效果。这些结果表明，MSCs在凝胶中的负载增强了它们的成骨能力，并改善了新骨的质量。因此，负载MSC的凝胶有望成为骨组织工程的有效治疗生物材料。版权所有(C)2012 John Wiley&Sons，Ltd.
The authors previously created HAp or CaCO3 formed on or in agarose gels (HAp and CaCO3 gels, respectively) as biocompatible and biodegradable bone graft materials. However, these gels have limitations for bone regeneration. Mesenchymal stromal cells (MSCs) have osteogenic potential and are considered useful for bone tissue engineering. The purpose of this study was to clarify the osteogenic abilities of MSCs loaded in HAp or CaCO3 gels (MSC/HAp and MSC/CaCO3 gels, respectively) using a rat cranial defect model compared to HAp and CaCO3 gels alone. HAp, CaCO3, MSC/Hap, and MSC/CaCO3 gels were prepared for in vivo analyses and implanted into full-thickness bone defects created in the rat cranium. All samples were assessed radiologically and histologically at 4 and 8 weeks after implantation. Using microfocus-computed tomography, an increase in bone formation was observed in the MSG-loaded gels compared to the gels alone. In addition, peripheral quantitative computed tomography revealed higher bone mineral contents in the MSC-loaded gels compared to the gels alone. After transmission X-ray diffraction analyses, the degree of apatite c-axis orientation as a bone quality index of newly formed bone in the MSC-loaded gels was close to that of living cranial bone. Histologically, more extensive bone formation was detected in the MSC-loaded gels compared to gels alone. Overall, MSC/HAp and MSC/CaCO3 gels showed equivalent efficacy for bone regeneration. These findings demonstrate that loading of MSCs into the gels strengthened their osteogenic ability and improved the quality of the newly formed bone. As a result, MSC-loaded gels could represent viable therapeutic biomaterials for bone tissue engineering. Copyright (C) 2012 John Wiley & Sons, Ltd.