Human fallopian tube expresses transforming growth factor (TGF beta) isoforms, TGF beta type I-III receptor messenger ribonucleic acid and protein, and contains [125I]TGF beta-binding sites.
Human fallopian tube expresses transforming growth factor (TGF beta) isoforms, TGF beta type I-III receptor messenger ribonucleic acid and protein, and contains [125I]TGF beta-binding sites.
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人输卵管表达转化生长因子 (TGF β) 同种型、TGF β I-III 型受体信使核糖核酸和蛋白质，并含有 [125I]TGF β 结合位点。
DOI：
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发表时间：
1994
影响因子：
5.8
通讯作者：
K. Flanders
中科院分区：
文献类型：
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作者：
Y. Zhao;N. Chegini;K. Flanders
人输卵管总核糖核酸（RNA）的逆转录-聚合酶链反应分析显示，转化生长因子β 1（TGF β 1）、TGF β 2和TGF β 3以及TGF β I-III型信使RNA（mRNA）在该组织中表达。使用TGF β亚型特异性[35 S]40-mer寡核苷酸探针和多克隆抗体进行的原位杂交和免疫组织化学观察表明，所有输卵管细胞类型均表达TGF β亚型和TGF β II型受体mRNA和蛋白。输卵管上皮细胞似乎比其他细胞类型表达更多的TGF β 1 mRNA和TGF β 1-3蛋白，而TGF β 2和TGF β 3 mRNA似乎在上皮细胞和其他输卵管细胞类型中同等表达。在上皮衬里中，壶腹和峡部区域的纤毛和非纤毛细胞似乎以相似的水平表达TGF β和TGF β II型受体的mRNA和蛋白。TGF β s在输卵管上皮细胞中的免疫染色强度在早期增殖和晚期分泌期低于中期至晚期增殖期和早期至中期分泌期的月经周期，并在绝经后期间减少。然而，在这些细胞中的免疫反应性TGF β II型受体的强度在周期中没有变化，如TGF β所见。[125 I]TGF β 1的定量放射自显影表明输卵管含有TGF β 1的特异性结合位点。每100微米2的净颗粒密度，计算不同的细胞类型，表明上皮细胞具有显着高于其他输卵管细胞类型的颗粒密度（P < 0.05），具有类似的密度在晚期增殖和早期分泌阶段的周期。这些结果提供了人类输卵管表达mRNA和蛋白质并含有TGF β系统的特异性结合位点的第一个证据，表明TGF β在各种输卵管功能中的自分泌/旁分泌作用。
Reverse transcription-polymerase chain reaction analysis of total ribonucleic acid (RNA) from human fallopian tubes revealed that transforming growth factor-beta 1 (TGF beta 1), TGF beta 2, and TGF beta 3 as well as TGF beta type I-III messenger RNA (mRNA) are expressed in this tissue. In situ hybridization and immunohistochemical observations using TGF beta isoform-specific [35S]40-mer oligonucleotide probes and polyclonal antibodies indicate that all the tubal cell types express TGF beta isoforms and TGF beta type II receptor mRNA and protein. The tubal epithelial cells appeared to express more TGF beta 1 mRNA and TGF beta 1-3 proteins than other cell types, whereas TGF beta 2 and TGF beta 3 mRNA appeared to be equally expressed in the epithelial and other tubal cell types. In the epithelial lining, both ciliated and nonciliated cells in the ampullary and isthmus regions appeared to express mRNA and protein for TGF beta s and TGF beta type II receptor at a similar level. The intensity of immunostaining of TGF beta s in tubal epithelial cells was lower during the early proliferative and late secretory than the mid- to late proliferative and early to midsecretory phases of the menstrual cycle and reduced during the postmenopausal period. However, the intensity of immunoreactive TGF beta type II receptor in these cells did not vary during the cycle as much as that seen with TGF beta s. Quantitative autoradiography of [125I]TGF beta 1 indicates that fallopian tubes contain specific binding sites for TGF beta 1. Net grain density per 100 microns 2, calculated for different cell types, indicates that the epithelial cells had a significantly higher grain density than other tubal cell types (P < 0.05), with similar densities in the late proliferative and early secretory phases of the cycle. These results provide the first evidence that human fallopian tubes express mRNA and protein and contain specific binding sites for TGF beta system, suggesting an autocrine/paracrine role for TGF beta in a variety of tubal functions.