Bmp2 Is Required for Odontoblast Differentiation and Pulp Vasculogenesis
Bmp2 Is Required for Odontoblast Differentiation and Pulp Vasculogenesis
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DOI：
10.1177/0022034511424409
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发表时间：
2012-01-01
影响因子：
7.6
通讯作者：
Gluhak-Heinrich, J.
中科院分区：
文献类型：
--
作者：
Yang, W.;Harris, M. A.;Gluhak-Heinrich, J.
使用Bmp 2 floxed/3.6Col1a1-Cre（Bmp 2-cKO（od））小鼠模型，我们观察到在早期极化成牙本质细胞中去除Bmp 2基因后，牙发生和牙本质形成严重缺陷。Bmp 2-cKO（od）中的成牙本质细胞不能正常成熟，不能与正常牙本质小管形成适当的牙本质，也不能激活终末分化，如Osterix、Col 1a 1和Dspp表达降低所反映的。牙本质较少，牙本质矿化不足，呈斑片状。我们还描述了成牙本质细胞中Bmp 2基因对牙髓中血管床和相关周细胞形成的间接影响。这种血管生态位和CD 146+周细胞的数量可能受成牙本质细胞中牙源性和Bmp 2依赖性VegfA产生的控制。Bmp 2的复杂作用，假设是直接和间接的，导致牙齿在整个生命中的永久性缺陷，并导致牙齿牙本质数量少和质量差。
Using the Bmp2 floxed/3.6Col1a1-Cre (Bmp2-cKO(od)) mouse model, we have observed severe defects in odontogenesis and dentin formation with the removal of the Bmp2 gene in early-polarizing odontoblasts. The odontoblasts in the Bmp2-cKO(od) do not mature properly and fail to form proper dentin with normal dentinal tubules and activate terminal differentiation, as reflected by decreased Osterix, Col1a1, and Dspp expression. There is less dentin, and the dentin is hypomineralized and patchy. We also describe an indirect effect of the Bmp2 gene in odontoblasts on formation of the vascular bed and associated pericytes in the pulp. This vascular niche and numbers of CD146+ pericytes are likely controlled by odontogenic and Bmp2-dependent VegfA production in odontoblasts. The complex roles of Bmp2, postulated to be both direct and indirect, lead to permanent defects in the teeth throughout life, and result in teeth with low quantities of dentin and dentin of poor quality.