Evidence for apoptosis of the majority of T cells activated in vitro with Actinobacillus actinomycetemcomitans.
Evidence for apoptosis of the majority of T cells activated in vitro with Actinobacillus actinomycetemcomitans.
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大多数 T 细胞在体外被 Actinobacillus actinomycetemcomitans 激活发生凋亡的证据。
DOI：
10.1034/j.1399-302x.2000.150504.x
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发表时间：
2000
影响因子：
--
通讯作者：
Zadeh,HH
中科院分区：
文献类型：
--
作者：
Nalbant,A;Zadeh,HH
我们之前的研究表明，用放线菌刺激的T细胞中，近一半在几个小时内被激活。然而，尚不清楚是否所有这些T细胞都能存活。本研究的目的是确定T细胞是否会对a产生反应。actinomycetemcomitansundergo细胞凋亡。为此，在a存在的不同时间点培养外周血单个核细胞。actinomycetemcomitans。流式细胞分析表明，暴露于a的制剂后。通过膜联蛋白V结合测量，放线菌细胞逐渐将其质膜磷脂酰丝氨酸外化。大约一半的T细胞在96小时内结合膜联蛋白V。在此期间，膜联蛋白V阳性的T细胞也结合了碘化丙啶，这表明膜完整性的丧失。磷脂酰丝氨酸外化在活化的（CD69+） T细胞中发生率更高，其中大约三分之二变为膜联蛋白v阳性。流式细胞术分析也显示膜联蛋白v阳性和碘化丙啶阳性T细胞萎缩。本文提供的数据为大多数对a有反应的T细胞诱导凋亡提供了证据。actinomycetemcomitans。
Our previous studies had demonstrated that nearly half of all T cells stimulated withActinobacillus actinomycetemcomitansare activated within a few hours. However, it was not known whether all of these T cells survive. The aim of the present study was to determine whether the T cells activated in response toA. actinomycetemcomitansundergo apoptosis. To that end, peripheral blood mononuclear cells were cultured at different time points in the presence ofA. actinomycetemcomitans. Flow cytometric analysis demonstrated that, following exposure to a preparation ofA. actinomycetemcomitans, T cells progressively externalized their plasma membrane phosphatidylserine, as measured by annexin V binding. Approximately half of all T cells bound annexin V by 96 h. During this period, Annexin V–positive T cells also incorporated propidium iodide suggesting loss of membrane integrity. The externalization of phosphatidylserine occurred at a higher rate among activated (CD69+) T cells, where roughly two‐thirds became Annexin V–positive. Flow cytometric analysis also demonstrated shrinkage of the Annexin V–positive and propidium iodide–positive T cells. The data presented here provides evidence for the induction of apoptosis among the majority of the T cells responding toA. actinomycetemcomitans.