Electrochemiluminescence immunosensor for tumor markers based on biological barcode mode with conductive nanospheres
Electrochemiluminescence immunosensor for tumor markers based on biological barcode mode with conductive nanospheres
复制标题
基于导电纳米球生物条码模式的肿瘤标志物电化学发光免疫传感器
DOI：
10.1016/j.bios.2013.09.041
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发表时间：
2014-03-15
影响因子：
12.6
通讯作者：
Wang, Sui
中科院分区：
文献类型：
--
作者：
Du, Shuping;Guo, Zhiyong;Wang, Sui
基于生物条形码模式，研制了一种新型的电化学发光免疫传感器，用于高灵敏度和高选择性的肿瘤标志物检测。将N-（4-氨基丁基）-N-乙基异鲁米诺（ABEI）和第二抗体（Ab（2））同时固定在导电纳米微球上，构建ABEI/Ab（2）-CNSs探针，该探针能与Ab（2）形成夹心免疫复合物，并能发射ABEI的电化学发光信号。在导电纳米球表面包覆一层金，可以有效地扩展ECL免疫传感器的外亥姆霍兹平面。得益于此，所有固定在导电纳米球上的ABEI分子都将作为生物条形码，在不干扰第二抗体活性的情况下提供原位ECL信号。在这种情况下，ECL免疫传感器的灵敏度将大大提高，因为抗原分子将对应于数千个ABEI分子的ECL信号。以前列腺特异性抗原（PSA）为模型肿瘤标志物，电化学发光强度随PSA浓度的对数增加而增加，线性范围为0.04 ~ 10 fg/mL。此外，该方法的特异性、稳定性、重现性、再生性和应用性均令人满意。因此，该电化学发光免疫传感器具有潜在的实际检测的疾病相关的蛋白质，除了在临床诊断中的肿瘤标志物。(C)2013爱思唯尔有限公司版权所有。
A novel sandwich-type electrochemiluminescence (ECL) immunosensor was developed for highly sensitive and selective determination of tumor markers based on biological barcode mode. N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and the second antibody (Ab(2)) were simultaneously immobilized on conductive nanospheres to construct ABEI/Ab(2)-CNSs probes, which could form sandwich immunocomplex by Ab(2) and emit ECL signals by ABEI. The gold layer coated on the surface of the conductive nanospheres could extend the outer Helmholtz plane (OHP) of the ECL immunosensor effectively. Benefited from it, all ABEI molecules immobilized on conductive nanospheres would act as biological barcode to give in-situ ECL signals without interfering with the activity of the second antibody. In such a case, the sensitivity of the ECL immunosensor would be greatly improved because an antigen molecule would correspond to ECL signals of thousands of ABEI molecules. Using prostate specific antigen (PSA) as a model tumor marker, the ECL intensity was found to increase with the logarithm of PSA concentration with a wide linear range from 0.04 to 10 fg/mL. In addition, specificity, stability, reproducibility, regeneration and application were satisfactory. Therefore, this developed ECL immunosensor has a potential for practical detection of disease-related proteins besides tumor markers in the clinical diagnostics. (C) 2013 Elsevier B.V. All rights reserved.