BRAF-activated LncRNA functions as a tumor suppressor in papillary thyroid cancer.
BRAF-activated LncRNA functions as a tumor suppressor in papillary thyroid cancer.
复制标题
BRAF 激活的 LncRNA 在甲状腺乳头状癌中发挥肿瘤抑制作用
DOI：
10.18632/oncotarget.10825
复制
发表时间：
2017-01-03
期刊：
影响因子：
--
通讯作者：
Ji QH
中科院分区：
文献类型：
--
作者：
Liao T;Qu N;Shi RL;Guo K;Ma B;Cao YM;Xiang J;Lu ZW;Zhu YX;Li DS;Ji QH
长链非编码rna （Long non-coding rna, lncRNAs）参与了癌细胞的发生、细胞周期调控、迁移、增殖、凋亡、转移和耐药等过程。braf激活的非编码RNA （BANCR）既是致癌基因，也是肿瘤抑制基因。在这里，我们通过使用qRT-PCR评估92例患者甲状腺乳头状癌（PTC）和匹配正常甲状腺上皮组织中的BANCR水平，研究了BANCR在PTC中的作用。我们还利用慢病毒载体构建PTC细胞系，研究BANCR过表达对癌细胞增殖、凋亡、迁移和侵袭的影响。我们的结果表明，PTC肿瘤组织中的BANCR水平低于对照组织。降低的BANCR水平与肿瘤大小、多灶性病变的存在和PTC的晚期相关。BANCR过表达可抑制PTC细胞增殖，促进细胞凋亡，抑制转移。它还灭活了ERK1/2和p38，用MEK抑制剂U0126处理后，这种效果得到增强。最后，在异种移植小鼠模型中，BANCR过表达显著抑制PTC细胞的肿瘤生长。这些结果表明，BANCR可抑制PTC的肿瘤发生，BANCR水平可作为一种新的预后指标。
Long non-coding RNAs (lncRNAs) participate in cancer cell tumorigenesis, cell cycle control, migration, proliferation, apoptosis, metastasis and drug resistance. The BRAF-activated non-coding RNA (BANCR) functions as both an oncogene and a tumor suppressor. Here, we investigated BANCR's role in papillary thyroid carcinoma (PTC) by assessing BANCR levels in PTC and matched normal thyroid epithelial tissues from 92 patients using qRT-PCR. We also used lentiviral vectors to establish PTC cell lines to investigate the effects of BANCR overexpression on cancer cell proliferation, apoptosis, migration and invasion. Our results indicate BANCR levels are lower in PTC tumor tissues than control tissues. Decreased BANCR levels correlate with tumor size, the presence of multifocal lesions and advanced PTC stage. BANCR overexpression reduced PTC cell proliferation and promoted apoptosis, which inhibited metastasis. It also inactivated ERK1/2 and p38, and this effect was enhanced by treatment with the MEK inhibitor U0126. Finally, BANCR overexpression dramatically inhibited tumor growth from PTC cells in xenograft mouse models. These results suggest BANCR inhibits tumorigenesis in PTC and that BANCR levels may be used as a novel prognostic marker.