Arabidopsis BPM Proteins Function as Substrate Adaptors to a CULLIN3-Based E3 Ligase to Affect Fatty Acid Metabolism in Plants
Arabidopsis BPM Proteins Function as Substrate Adaptors to a CULLIN3-Based E3 Ligase to Affect Fatty Acid Metabolism in Plants
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DOI：
10.1105/tpc.112.107292
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发表时间：
2013-06-01
期刊：
影响因子：
11.6
通讯作者：
Hellmann, Hanjo
中科院分区：
文献类型：
--
作者：
Chen, Liyuan;Lee, Joo Hyun;Hellmann, Hanjo
转录过程的调控是一种关键的机制，能够有效地协调所需蛋白质的合成，以响应环境和细胞的变化。转录因子需要精确的活性调控，因为它们是关键的调节因子，确保靶基因的特异性表达。在这项工作中，我们证明了基于CULLIN3的E3连接酶具有与广泛的乙烯反应因子(ERF)/APETALA2(AP2)转录因子相互作用的潜力，这种作用是由MATH-BTB/POZ(针对Meprin和TRAF[肿瘤坏死因子受体相关因子]同系物)-BRoad Complex，TramTrack，Bric-a-brac/Pox Virus和Zincinger)蛋白介导的。与E3连接酶的组装通过26S蛋白酶体导致其底物的降解，如WRINKLED1ERF/AP2蛋白所证明的那样。此外，MATH-BTB/POZ蛋白的缺失广泛影响植物的发育，并导致突变种子中脂肪酸含量的变化。总之，这项工作证明了植物中脂肪酸代谢和E3连接酶活性之间的联系，并建立了基于CUL3的E3连接酶作为涉及ERF/AP2家族成员的转录过程的关键调节因子。
Regulation of transcriptional processes is a critical mechanism that enables efficient coordination of the synthesis of required proteins in response to environmental and cellular changes. Transcription factors require accurate activity regulation because they play a critical role as key mediators assuring specific expression of target genes. In this work, we show that CULLIN3-based E3 ligases have the potential to interact with a broad range of ETHYLENE RESPONSE FACTOR (ERF)/APETALA2 (AP2) transcription factors, mediated by MATH-BTB/POZ (for Meprin and TRAF [tumor necrosis factor receptor associated factor] homolog)-Broad complex, Tramtrack, Bric-a-brac/Pox virus and Zinc finger) proteins. The assembly with an E3 ligase causes degradation of their substrates via the 26S proteasome, as demonstrated for the WRINKLED1 ERF/AP2 protein. Furthermore, loss of MATH-BTB/POZ proteins widely affects plant development and causes altered fatty acid contents in mutant seeds. Overall, this work demonstrates a link between fatty acid metabolism and E3 ligase activities in plants and establishes CUL3-based E3 ligases as key regulators in transcriptional processes that involve ERF/AP2 family members.