Insecticide resistance monitoring of field-collected Anopheles gambiae s.l. populations from Jinja, eastern Uganda, identifies high levels of pyrethroid resistance
Insecticide resistance monitoring of field-collected Anopheles gambiae s.l. populations from Jinja, eastern Uganda, identifies high levels of pyrethroid resistance
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DOI：
10.1111/j.1365-2915.2012.01055.x
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发表时间：
2013-09-01
影响因子：
1.9
通讯作者：
Donnelly, M. J.
中科院分区：
文献类型：
--
作者：
Mawejje, H. D.;Wilding, C. S.;Donnelly, M. J.
疟疾媒介冈比亚按蚊（Anopheles gambiae s.l.）（双翅目：蚊科）威胁到以蚊类为基础的防治工作，需要定期监测。我们评估了现场收集的安。冈比亚公司使用世界卫生组织（WHO）生物测定法从乌干达金贾获得。只有安。冈比亚公司和一个.阿拉伯亚种（70%）。女安。冈比亚表现出极高的拟除虫菊酯抗性（氯菊酯LT 50> 2 h;溴氰菊酯LT 50> 5 h）。女安。阿拉伯按蚊对氯菊酯具有抗性，对溴氰菊酯的敏感性降低。然而，虽然安。冈比亚按蚊对DDT具有抗性; arabiensis完全感病。这两个物种是完全敏感的苯虫威和杀螟硫磷。Kdr 1014 S在安的金贾人口中迅速增加。冈比亚公司并且现在接近固定（95%），这与酶介导的选择一致，但是目前在An中处于低频率。阿拉伯藜（0.07%）。Kdr 1014 F在安氏按蚊中的频率也很低。冈比亚。这些频率排除了与表型耐药相关性的充分把握度试验。PBO生物测定导致拟除虫菊酯敏感性几乎完全恢复，表明CYP 450参与抗性。少数（0.22%）的安。冈比亚公司xAn。发现了arabiensis杂种，表明种间抗性等位基因渐渗的可能性。在金贾发现的对拟除虫菊酯的高度抗药性可能会降低病媒控制方案的效力，这些方案依赖于拟除虫菊酯浸渍蚊帐或室内喷洒拟除虫菊酯。
Insecticide resistance in the malaria vector Anopheles gambiae s.l. (Diptera: Culicidae) threatens insecticide-based control efforts, necessitating regular monitoring. We assessed resistance in field-collected An. gambiae s.l. from Jinja, Uganda using World Health Organization (WHO) biosassays. Only An. gambiae s.s. and An. arabiensis (70%) were present. Female An. gambiae exhibited extremely high pyrethroid resistance (permethrin LT50 > 2 h; deltamethrin LT50 > 5 h). Female An. arabiensis were resistant to permethrin and exhibited reduced susceptibility to deltamethrin. However, while An. gambiae were DDT resistant, An. arabiensis were fully susceptible. Both species were fully susceptible to bendiocarb and fenitrothion. Kdr 1014S has increased rapidly in the Jinja population of An. gambiae s.s. and now approaches fixation (95%), consistent with insecticide-mediated selection, but is currently at a low frequency in An. arabiensis (0.07%). Kdr 1014F was also at a low frequency in An. gambiae. These frequencies preclude adequately-powered tests for an association with phenotypic resistance. PBO synergist bioassays resulted in near complete recovery of pyrethroid susceptibility suggesting involvement of CYP450s in resistance. A small number (0.22%) of An. gambiae s.s. xAn. arabiensis hybrids were found, suggesting the possibility of introgression of resistance alleles between species. The high levels of pyrethroid resistance encountered in Jinja threaten to reduce the efficacy of vector control programmes which rely on pyrethroid-impregnated bednets or indoor spraying of pyrethroids.