Parallel, Large-Scale, and Long Synthetic Oligodeoxynucleotide Purification Using the Catching Full-Length Sequence by Polymerization Technique
Parallel, Large-Scale, and Long Synthetic Oligodeoxynucleotide Purification Using the Catching Full-Length Sequence by Polymerization Technique
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DOI：
10.1021/acs.oprd.8b00209
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发表时间：
2018-09-01
影响因子：
3.4
通讯作者：
Fang, Shiyue
中科院分区：
文献类型：
--
作者：
Eriyagama, Dhananjani N. A. M.;Shahsavari, Shahien;Fang, Shiyue
聚合捕集法合成寡脱氧核苷酸（oligodeoxynucleotide， ODN）纯化技术具有同时纯化12个寡脱氧核苷酸的高通量纯化潜力，便于在60 μ mol合成规模下进行大规模纯化，对长达303-mers的寡脱氧核苷酸具有很强的纯化能力。LC-MS分析表明，该技术纯化的odn具有良好的纯度。
The catching by polymerization synthetic oligodeoxynucleotide (ODN) purification technique was shown to be potentially suitable for high-throughput purification by the purification of 12 ODNs simultaneously, to be convenient for large-scale purification by purification at the 60 mu mol synthesis scale, and to be highly powerful for purification of long ODNs by purification of ODNs as long as 303-mers. LC-MS analysis indicated that the ODNs purified with the technique have excellent purity.