The effect of Mongolian medical acupuncture on cytokines and neurotransmitters in the brain tissue of insomniac rats
The effect of Mongolian medical acupuncture on cytokines and neurotransmitters in the brain tissue of insomniac rats
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蒙医针灸对失眠大鼠脑组织细胞因子和神经递质的影响
DOI：
10.1016/j.eujim.2015.05.008
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发表时间：
2015-10-01
影响因子：
2.5
通讯作者：
Agula, B.
中科院分区：
文献类型：
--
作者：
Si, Lengge;Wang, Yuehong;Agula, B.
前言：蒙医针灸（MMA）被认为对失眠有显著的治疗效果。方法：SD大鼠随机分为6组（对照1组、对照2组、正常针刺组、失眠组、失眠+针刺组、失眠+西药组），分别腹腔注射对氯苯丙氨酸建立失眠模型。针刺“定会、合益、心"穴，观察戊巴比妥钠致失眠大鼠的自主活动及睡眠时间。采用酶联免疫吸附法测定脑前额叶皮质、下丘脑和海马组织中白细胞介素（IL）-1，IL-2和IL-6以及肿瘤坏死因子（TNF）-α的水平。采用高效液相色谱-电子捕获检测器（HPLC-ECD）测定去甲肾上腺素（NE）、多巴胺（DA）、5-羟色胺（5-HT）、谷氨酸（Glu）和γ-氨基丁酸（GABA）的含量，采用高效液相色谱-反相电子捕获检测器（HPLC-RE-ECD）测定乙酰胆碱（Ach）的含量。MMA显著延长戊巴比妥钠诱导的睡眠时间。IL-1、IL-2、IL-6和TNF-α水平较失眠模型组显著升高（P
Introduction: Mongolian medical acupuncture (MMA) is thought to have a significant therapeutic effect on insomnia. The effect of MMA on levels of interleukins (ILs) and neurotransmitters in insomniac rats was investigated and the potential mechanism operating for insomnia is discussed.Methods: SD rats in 6 groups (control group 1, control group 2, normal acupuncture group, insomnia group, insomnia + acupuncture group, and insomnia + Western medicine group) were intraperitoneally injected with p-chlorophenylalanine to provide an insomniac model. "Dinghui, Heyi and Xin acupoints" were stimulated with needles to observe the locomotor activities of insomniac rats (injected with pentobarbital sodium) and their sleep time. Levels of interleukin (IL)-1, IL-2, and IL-6, and tumor necrosis factor (TNF)-alpha in the brain prefrontal lobe cortex, hypothalamus, and hippocampus tissue were measured by enzyme linked immunosorbent assay. Noradrenaline (NE), dopamine (DA), 5-hydroxytryptamine (5-HT), glutamic acid (Glu) and gamma-aminobutyric acid (GABA) levels were measured by HPLC-ECD, and acetyl choline (Ach) level was determined by HPLC-RE-ECD.Results: Insomniac rats experienced quietness, reduced activities and recovery of sleep time, drinking and eating and mental state after MMA stimulation. MMA significantly prolonged the sleep time induced by pentobarbital sodium. Levels of IL-1, IL-2, IL-6 and TNF-a significantly increased compared with those of insomniac models (P