ELONGATION OF DISPLACEMENT-LOOP STRANDS IN HUMAN AND MOUSE MITOCHONDRIAL-DNA IS ARRESTED NEAR SPECIFIC TEMPLATE SEQUENCES
ELONGATION OF DISPLACEMENT-LOOP STRANDS IN HUMAN AND MOUSE MITOCHONDRIAL-DNA IS ARRESTED NEAR SPECIFIC TEMPLATE SEQUENCES
复制标题
DOI：
10.1073/pnas.78.10.6116
复制
发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
CLAYTON, DA
中科院分区：
文献类型：
--
作者：
DODA, JN;WRIGHT, CT;CLAYTON, DA
动物线粒体[mt] DNA在重链复制起点处维持置换环（D环）。这些D环代表了重链合成的严格限制，并提供了一个独特的机会来检查DNA合成的终止。在核苷酸水平上的直接大小测定表明，人和小鼠mtDNA的D环链的3“末端是离散的，并在互补模板链上的3-5个核苷酸内定位。就人类线粒体DNA而言，在15个核苷酸模板序列（3”T-A-A-C-C-C-A-A-A-A-A-A-A-T-A-C-A 5”）下游51-53个核苷酸处有一个单一的三核苷酸终止点，该终止点在小鼠线粒体DNA D环区3”（T-A-A-Py-Py-A-A-A-T-T-A-C-A 5”）中重复4次。5个主要小鼠D-环链的终止点是4个重复模板序列下游的24-63个核苷酸。D-环链延伸的停滞是由模板序列决定的事件。
Animal mitochondrial [mt] DNA maintains a displacement loop (D loop) at the heavy strand origin of replication. These D loops represent sharply limited synthesis of heavy strands and provide a unique opportunity to examine the termination of DNA synthesis. Direct sizing at the nucleotide level indicates that the 3'' ends of D-loop strands of human and mouse mtDNA are discrete and map within 3-5 nucleotides on the complementary template strand. In the case of human mtDNA, there is a single trinucleotide stop point 51-53 nucleotides downstream from a 15-nucleotide template sequence (3''T-A-A-C-C-C-A-A-A-A-A-T-A-C-A 5'') which is repeated 4 times in the mouse mtDNA D loop region 3''(T-A-A-Py-Py-A-A-A-T-T-A-C-A 5''). The stop points of the 5 major mouse D-loop strands are 24-63 nucleotides downstream from the 4 repeated template sequences. The arrest of D-loop strand elongation is an event determined by template sequence.