Seed Fatty Acid Reducer acts downstream of gibberellin signalling pathway to lower seed fatty acid storage in Arabidopsis
Seed Fatty Acid Reducer acts downstream of gibberellin signalling pathway to lower seed fatty acid storage in Arabidopsis
复制标题
种子脂肪酸还原剂作用于赤霉素信号通路下游，降低拟南芥种子脂肪酸储存
DOI：
10.1111/j.1365-3040.2012.02546.x
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发表时间：
2012-12-01
影响因子：
7.3
通讯作者：
Jiang, Lixi
中科院分区：
文献类型：
--
作者：
Chen, Mingxun;Du, Xue;Jiang, Lixi
先前基于微阵列分析的研究已经发现，DELLAs下调未开放的花和/或吸胀的种子中的几个GDSL基因。这表明DELLA在种子脂肪酸（FA）代谢中的作用。在本研究中，增强赤霉素（GA）信号通过DELLA突变或外源赤霉素酸A3（GA 3）导致上调表达的转录因子的胚胎发生和种子发育，参与FA生物合成途径的基因，和5个GDSL型种子脂肪酸还原酶（SFAR）基因。SFAR过表达降低了种子总FA含量，并导致种子FA组成的特定模式。这种SFAR足迹也可以在具有增强的GA 3信号传导的植物中发现。相反，SFAR功能的丧失显著增加种子FA含量。过表达SFAR的转基因株系对胁迫环境不太敏感，与野生型（WT）植物相比，其发芽率更高，幼苗建立更好。GDSL型水解蛋白是一个在拟南芥中很大程度上未被表征的蛋白质家族。它们的生物学功能仍然知之甚少。SFAR减少种子FA储存并作用于GA信号传导途径的下游。我们提供的第一个证据表明，一些GDSL蛋白在某种程度上参与了拟南芥种子中的FA降解。
Previous studies based on microarray analysis have found that DELLAs down-regulate several GDSL genes in unopened flowers and/or imbibed seeds. This suggests the role of DELLAs in seed fatty acid (FA) metabolism. In the present study, enhancement of gibberellin (GA) signalling through DELLA mutation or exogenous gibberellin acid A3 (GA3) resulted in the up-regulated expression of transcription factors for embryogenesis and seed development, genes involved in the FA biosynthesis pathway, and five GDSL-type Seed Fatty Acid Reducer (SFAR) genes. SFAR overexpression reduced the total seed FA content and led to a particular pattern of seed FA composition. This SFAR footprint can also be found in plants with enhanced GA3 signalling. By contrast, the loss of SFAR function dramatically increases the seed FA content. The transgenic lines that overexpress SFAR were less sensitive to stressful environments, reflected by a higher germination rate and better seedling establishment compared with the wild type (WT) plants. The GDSL-type hydrolyzer is a family of proteins largely uncharacterized in Arabidopsis. Their biological function remains poorly understood. SFAR reduces seed FA storage and acts downstream of the GA signalling pathway. We provide the first evidence that some GDSL proteins are somehow involved in FA degradation in Arabidopsis seeds.