Increased accumulation of CD30 ligand-positive mast cells associates with eosinophilic inflammation in nasal polyps
Increased accumulation of CD30 ligand-positive mast cells associates with eosinophilic inflammation in nasal polyps
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CD30配体阳性肥大细胞积累增加与鼻息肉中的嗜酸性粒细胞炎症相关
DOI：
10.1002/lary.27658
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发表时间：
2019
期刊：
影响因子：
2.6
通讯作者：
Liu Zheng
中科院分区：
文献类型：
--
作者：
Zhai Guan-Ting;Li Jing-Xian;Zhang Xin-Hao;Liao Bo;Lu Xiang;Liu Zheng
慢性鼻窦炎伴鼻息肉（CRSwNP）患者肥大细胞活化与嗜酸性粒细胞炎症相关。在CRSwNP中，除了免疫球蛋白E之外的疾病特异性肥大细胞触发机制知之甚少。CD 30 L/CD 30是肿瘤坏死因子/受体超家族成员，对肥大细胞具有免疫调节功能。本研究旨在探讨CD 30和CD 30 L在CRSwNP中的表达及其功能。方法采用真实的实时荧光定量聚合酶链反应（RT-PCR）方法检测CRSwNP中CD 30和CD 30 L的mRNA表达。免疫荧光染色检测细胞内CD 30 L的表达。采用酶联免疫吸附法检测鼻黏膜组织中可溶性CD 30水平。结果与对照组相比，嗜酸性息肉中CD 30 mRNA表达水平升高，可溶性CD 30蛋白表达水平在嗜酸性息肉和非嗜酸性息肉中均升高，嗜酸性息肉中的表达水平升高更明显。鼻息肉组织中T细胞和B细胞均表达CD 30。与对照组相比，嗜酸性息肉中CD 30 L mRNA表达水平和CD 30 L+细胞和CD 30 L+类胰蛋白酶+肥大细胞数量增加，但非嗜酸性息肉中未增加。肥大细胞占嗜酸性息肉中CD 30 L+细胞的60%。CD 30诱导HMC-1细胞产生白细胞介素（IL）-4和IL-13而不脱粒。肥大细胞在嗜酸性息肉中表达IL-4和IL-13。结论CD 30/CD 30 L介导的肥大细胞活化可能促进CRSwNP的嗜酸性炎症反应.Level of Evidence NAL aryngoscope，129：E110-E117，2019
ObjectiveActivation of mast cells associates with eosinophilic inflammation in chronic rhinosinusitis with nasal polyps (CRSwNP). The disease‐specific mast cell‐triggering mechanisms apart from immunoglobulin E are poorly understood in CRSwNP. CD30L/CD30 are members of the tumor necrosis factor/receptor superfamily and display immune modulatory function on mast cells. The aim of this study was to explore the expression and function of CD30 and CD30L in CRSwNP.MethodsThe mRNA expression of CD30 and CD30L was analyzed by real‐time polymerase chain reaction. The cellular expression of CD30L was determined by immunofluorescence staining. The soluble CD30 levels in nasal tissues were detected by enzyme‐linked immunosorbent assay. HMC‐1 cells, a human mast cell line, were cultured and stimulated with CD30.ResultsCompared with control tissues, CD30 mRNA expression levels were increased in eosinophilic polyps, and soluble CD30 protein levels were upregulated in both eosinophilic and noneosinophilic polyps with a greater increase in eosinophilic type. CD30 was expressed by T cells and B cells in nasal polyps. The CD30L mRNA expression levels and the number of CD30L+cells and CD30L+tryptase+mast cells were increased in eosinophilic polyps but not in noneosinophilic polyps as compared with control tissues. Mast cells accounted for 60% of CD30L+cells in eosinophilic polyps. CD30 induced HMC‐1 cells to produce interleukin (IL)‐4 and IL‐13 without degranulation. Mast cells expressed IL‐4 and IL‐13 in eosinophilic polyps. The number of CD30L+tryptase+mast cells was positively correlated with the number of eosinophils and total inflammatory cells in eosinophilic polyps.ConclusionCD30/CD30L‐mediated mast cell activation may promote the eosinophilic inflammation in CRSwNP.Level of EvidenceNALaryngoscope, 129:E110–E117, 2019