Minor structural protein VP2 in rabbit hemorrhagic disease virus downregulates the expression of the viral capsid protein VP60
Minor structural protein VP2 in rabbit hemorrhagic disease virus downregulates the expression of the viral capsid protein VP60
复制标题
兔出血性疾病病毒次要结构蛋白VP2下调病毒衣壳蛋白VP60的表达
DOI：
10.1099/vir.0.015081-0
复制
发表时间：
2009-12-01
影响因子：
3.8
通讯作者：
Chen, Jianping
中科院分区：
文献类型：
--
作者：
Chen, Liu;Liu, Guangqing;Chen, Jianping
Rabbit hemorrhagic disease virus (RHDV) has two structural proteins: the major capsid protein VP60 and the minor capsid protein VP2. VP2 is speculated to play an important role in the virus life cycle. To investigate the effect of VP2 on VP60 expression, three types of experiment (baculovirus-insect cell system, mammalian-lucif erase assay system and in vitro coupled transcription/translation system) were used to express VP60 alone or co-expressed with VP2. Both forms of VP60 were able to form virus-like particles in insect cells. Western blot analysis and dual-luciferase assays demonstrated that the presence of VP2 results in clownregulation of the expression of VP60 in vivo. Real-time RT-PCR of mRNA levels showed that clownregulation of VP60 occurs at the transcriptional level. The ability of the viral minor structural protein VP2 to regulate capsid protein levels may contribute to effective virus infection.
