Identification and characterization of differentially expressed cDNAs of the vector mosquito, Anopheles gambiae
Identification and characterization of differentially expressed cDNAs of the vector mosquito, Anopheles gambiae
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DOI：
10.1073/pnas.93.23.13066
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发表时间：
1996-11-12
影响因子：
11.1
通讯作者：
Louis, C
中科院分区：
文献类型：
--
作者：
Dimopoulos, G;Richman, A;Louis, C
冈比亚按蚊在发育过程中差异表达的基因的分离和研究，特别是在与疟原虫的成熟和传播相关的组织中差异表达的基因，对于阐明媒介-寄生虫相互作用的基本分子机制是重要的。我们已经使用差异显示技术筛选在成年雄性、雌性以及吸血和未进食雌性的中肠组织中特异表达的mRNA，我们还筛选了在细菌感染的幼虫期蚊子后特异性诱导的mRNA，我们已经表征了19种不同的cDNA，其中大多数在蚊子生命周期中不具有发育调节的表达特异性，最令人感兴趣的是六种新的在成虫中肠特异性的序列，其中三种也受到血液喂养的调节。肠特异性序列编码麦芽糖酶，V-ATP酶亚基，后一个序列在细菌攻击下也能被诱导表达，除线粒体DNA片段外，其余18个序列构成表达的基因组序列标签，其中4个已被细胞遗传学定位。
The isolation and study of Anopheles gambiae genes that are differentially expressed in development, notably in tissues associated with the maturation and transmission of the malaria parasite, is important for the elucidation of basic molecular mechanisms underlying vector-parasite interactions, We have used the differential display technique to screen for mRNAs specifically expressed in adult males, females, and midgut tissues of blood-fed and unfed females, me also screened for mRNAs specifically induced upon bacterial infection of larval stage mosquitoes, We have characterized 19 distinct cDNAs, most of which shaw developmentally regulated expression specificity during the mosquito life cycle, The most interesting are six new sequences that are midgut-specific in the adult, three of which are also modulated by blood-feeding, The gut-specific sequences encode a maltase, a V-ATPase subunit, a GTP binding protein, two different lectins, and a nontrypsin serine protease, The latter sequence is also induced in larvae subjected to bacterial challenge, With the exception of a mitochondrial DNA fragment, the other 18 sequences constitute expressed genomic sequence tags, 4 of which have been mapped cytogenetically.