Microarray analysis of the effects of a gamma-protein kinase C null mutation on gene expression in striatum: a role for transthyretin in mutant phenotypes.
Microarray analysis of the effects of a gamma-protein kinase C null mutation on gene expression in striatum: a role for transthyretin in mutant phenotypes.
复制标题
γ-蛋白激酶 C 无效突变对纹状体基因表达影响的微阵列分析：运甲状腺素蛋白在突变表型中的作用。
DOI：
10.1007/s10519-006-9083-6
复制
发表时间：
2006
影响因子：
2.6
通讯作者：
Wehner,JeanneM
中科院分区：
文献类型：
--
作者：
Smith,AmyM;Bowers,BarbaraJ;Radcliffe,RichardA;Wehner,JeanneM
A constitutive null mutation of the neural-specific isotype of protein kinase C (γ-PKC) in mice produces alterations in behavioral traits and responses to ethanol suggesting that γ-PKC-mediated phosphorylation is essential for regulation of some behaviors. However, it is possible that some of the effects of γ-PKC gene deletion also may be due to altered gene expression. To examine alterations in gene expression, microarray analyses were performed on striatal tissue from wild types and mutants. A total of 143 genes and ESTs were identified as potential candidates related to differences between null mutants and wild types. Confirmation studies using qRT-PCR indicated that the expression of transthyretin was increased about 8-fold in striatum of naïve mutants compared to wild types. The effect of chronic ethanol treatment on transthyretin expression was analyzed because γ-PKC mutants do not develop tolerance to chronic ethanol treatment. Ethanol treatment of mutants reversed the dramatic increase in transthyretin expression seen in naïve and control-diet treated mutants, but did not affect transthyretin expression in wild types.
