THE TISSUE ARCHITECTURE OF SYNOVIAL MEMBRANES IN INFLAMMATORY AND NON-INFLAMMATORY JOINT DISEASES .1. THE LOCALIZATION OF THE MAJOR SYNOVIAL CELL-POPULATIONS AS DETECTED BY MONOCLONAL REAGENTS DIRECTED TOWARDS IA AND MONOCYTE-MACROPHAGE ANTIGENS
THE TISSUE ARCHITECTURE OF SYNOVIAL MEMBRANES IN INFLAMMATORY AND NON-INFLAMMATORY JOINT DISEASES .1. THE LOCALIZATION OF THE MAJOR SYNOVIAL CELL-POPULATIONS AS DETECTED BY MONOCLONAL REAGENTS DIRECTED TOWARDS IA AND MONOCYTE-MACROPHAGE ANTIGENS
复制标题
DOI：
10.1007/bf00541597
复制
发表时间：
1983-01-01
影响因子：
4
通讯作者：
MOHR, W
中科院分区：
文献类型：
--
作者：
BURMESTER, GR;LOCHER, P;MOHR, W
利用针对单核细胞-巨噬细胞谱系抗原和Ia抗原的单克隆试剂，研究了从非炎性关节疾病患者和类风湿性关节炎患者获得的滑膜的组织结构。重点放在I型、II型和III型滑膜细胞的定位上，这些滑膜细胞以前是根据单核细胞-巨噬细胞谱系（Mθ）和Ia抗原表达方面的细胞表面表型以及它们的吞噬能力或产生糖胺聚糖的能力来定义的。在非炎性关节疾病患者中，具有Mθ+Ia+（I型）表型的细胞构成了紧邻关节腔的滑膜细胞的大部分;具有该表型的细胞也分散在滑膜下组织和血管周围区域。III型成纤维细胞样细胞（Mθ和Ia抗原均不存在）是该患者滑膜下组织中的主要细胞群。在类风湿性关节炎患者中，Ia+Mθ+细胞以特征性的双重构型存在，在关节内间隙附近形成强阳性层，随后是Ia−Mθ−层，再次是强Ia+Mθ+层。在弥漫性炎症的滑膜下组织、血管周围区域以及淋巴浸润周围和淋巴浸润内也发现大量携带Mθ+Ia+抗原的滑膜细胞。通过Ia抗原的存在而不存在Mθ抗原来确定II型细胞的定位，这表明II型细胞主要位于滑膜细胞的增宽层、血管周围区域和淋巴浸润内。虽然在非炎性关节疾病患者中内皮细胞缺乏Ia和Mθ抗原，但在一些类风湿样本中鉴定出Ia+内皮细胞;然而，在该患者组中大多数内皮细胞也是Ia阴性的。
Utilizing monoclonal reagents directed towards antigens of the monocyte-macrophage lineage and Ia antigens, the tissue architecture of synovial membranes obtained from patients with non-inflammatory joint diseases and patients with rheumatoid arthritis was studied. Emphasis was placed on the localization of the type I, type II and type III synoviocytes that previously had been defined by their cell surface phenotype with regard to the expression of monocyte-macrophage lineage (Mθ) and Ia antigens as well as by their phagocytic capacity or the ability to produce glycosaminoglycans. In patients with non-inflammatory joint diseases, cells with the Mθ+Ia+(type I) phenotype constituted the majority of synoviocytes immediately adjacent to the joint cavity; cells with this phenotype were also scattered in the subsynovial tissue and in the perivascular regions. The fibroblastoid type III cells defined by the absence of both Mθ and Ia antigens formed the major cell population in the subsynovial tissue in this patient group. In patients with rheumatoid arthritis, the Ia+Mθ+cells were present in a characteristic double configuration forming an intensely positive layer adjacent to the intra-articular space followed by an Ia−Mθ−layer that again was succeeded by an intensely Ia+Mθ+layer. Large numbers of synoviocytes bearing Mθ+Ia+antigens were also demonstrated in the diffusely inflamed sub-synovial tissue, in the perivascular regions as well as around and within lymphoid infiltrates. The localization of type II cells defined by the presence of Ia antigens, but the absence of Mθ antigens was indicated to be primarily in the broadened layer of synoviocytes, in the perivascular regions and within lymphoid infiltrations. While endothelial cells lacked Ia and Mθ antigens in patients with non-inflammatory joint diseases, Ia+endothelial cells were identified in some rheumatoid samples; however, the majority of endothelial cells was also Ia negative in this patient group.