TH cells primed during influenza virus infection provide help for qualitatively distinct antibody responses to subsequent immunization.
TH cells primed during influenza virus infection provide help for qualitatively distinct antibody responses to subsequent immunization.
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DOI：
10.4049/jimmunol.163.9.4673
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发表时间：
1999-11
影响因子：
4.4
通讯作者：
D. Marshall;R. Sealy;M. Sangster;C. Coleclough
中科院分区：
文献类型：
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作者：
D. Marshall;R. Sealy;M. Sangster;C. Coleclough
病毒复制能力强烈影响小鼠颈部和纵隔淋巴结对流感病毒的原代抗体形成细胞(AFC)应答的质量。免疫球蛋白分泌物在纵隔淋巴结对感染性病毒的早期AFC反应中占主导地位，而在同一小鼠的颈淋巴结中，免疫球蛋白G在同型转换的AFC中的表达更为频繁；在对灭活病毒的反应中，这一模式相反。流感病毒A/波多黎各/8/34(A/PR8)和A/X-31共享8个基因组片段中的6个，只是血凝素(A/PR8中的H1，A/X-31中的H3)和神经氨酸酶(A/PR8中的N1，A/X-31中的N2)基因不同。因此，这些病毒会引起广泛的交叉反应TH群体，尽管它们的糖蛋白在血清学上是无关的。因此，从A/X-31感染中恢复的小鼠在受到A/PR8糖蛋白攻击时，会产生针对A/PR8糖蛋白的初级B细胞反应，尽管这种反应可以召唤记忆TH细胞。为了评估记忆TH群体对初级抗体反应的影响，我们比较了幼鼠和清除了A/X-31感染的小鼠对灭活的A/PR8的AFC反应。用灭活的A/PR8滴鼻免疫A/X-31免疫小鼠，其对A/PR8 H1和N1糖蛋白的AFC反应比未免疫的小鼠更强。然而，A/X-31免疫的小鼠淋巴结中H1/N1特异性AFC的亚型分布与未接种的小鼠相似。显然，在这种功能背景下，记忆TH细胞保留了帮助抗体反应的能力，这些反应在质量上不同于它们在主要反应中产生的反应。
The quality of the primary Ab-forming cell (AFC) response in cervical lymph nodes and mediastinal lymph nodes of mice to intranasal influenza virus was strongly influenced by viral replicative capacity. IgA secretors were prominent in the early AFC response to infectious virus in mediastinal lymph nodes, while IgG expression was more frequent among isotypically switched AFC in cervical lymph nodes of the same mice; this pattern was reversed in the response to inactivated virus. Influenza viruses A/Puerto Rico/8/34 (A/PR8) and A/X-31 share six of eight genome segments, differing only in hemagglutinin (H1 in A/PR8, H3 in A/X-31) and neuraminidase (N1 in A/PR8, N2 in A/X-31) genes. These viruses therefore elicit extensively cross-reactive TH populations, though their glycoproteins are serologically unrelated. Mice recovered from an A/X-31 infection thus mount a primary B cell response against A/PR8 glycoproteins, when challenged with the latter virus, though this response can call upon memory TH cells. To assess the impact of memory TH populations on a primary Ab response, we compared the AFC response to inactivated A/PR8 in naive mice and mice that had cleared an A/X-31 infection. A/X-31 immune mice mounted a more vigorous AFC response against A/PR8 H1 and N1 glycoproteins than naive animals, when immunized intranasally with inactivated A/PR8. However the distribution of isotypes among H1/N1-specific AFC in lymph nodes of A/X-31-primed mice resembled that of naive mice. Evidently, in this functional context, memory TH cells retained the ability to help Ab responses different in quality from that generated during their primary reaction.