Using Looming Visual Stimuli to Evaluate Mouse Vision.
Using Looming Visual Stimuli to Evaluate Mouse Vision.
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DOI：
10.3791/59766
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发表时间：
2019-06
期刊：
影响因子：
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通讯作者：
Christina C. Koehler;Leo M Hall;Chase B. Hellmer;T. Ichinose
中科院分区：
文献类型：
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作者：
Christina C. Koehler;Leo M Hall;Chase B. Hellmer;T. Ichinose
中枢神经系统中的视觉系统处理各种视觉信号。虽然从视网膜到外侧膝状体核到视觉皮层的整体结构已经被表征，但是该系统是复杂的。已经进行了细胞和分子研究来阐明视觉处理的基础机制，并通过扩展来阐明疾病机制。这些研究可能有助于人工视觉系统的发展。为了验证这些研究的结果，行为视觉测试是必要的。在这里，我们表明，若隐若现的刺激实验是一个可靠的小鼠视觉测试，需要一个相对简单的设置。这项若隐若现的实验是在一个大的封闭空间里进行的，一个角落里有一个避难所，天花板上有一个电脑显示器。计算机显示器旁边的CCD摄像机用于观察小鼠行为。将小鼠置于围栏中10分钟，并使其适应和探索周围环境。然后，显示器投射一个程序衍生的隐约刺激10次。老鼠对刺激的反应要么是僵住，要么是逃到藏身之处。记录了小鼠在出现刺激前后的行为，并使用运动跟踪软件分析了视频。在隐现刺激后，小鼠运动的速度显著改变。相比之下，在盲小鼠中未观察到反应。我们的研究结果表明，简单的隐现实验是一个可靠的测试小鼠的视觉。
The visual system in the central nervous system processes diverse visual signals. Although the overall structure has been characterized from the retina through the lateral geniculate nucleus to the visual cortex, the system is complex. Cellular and molecular studies have been conducted to elucidate the mechanisms underpinning visual processing and, by extension, disease mechanisms. These studies may contribute to the development of artificial visual systems. To validate the results of these studies, behavioral vision testing is necessary. Here, we show that the looming stimulation experiment is a reliable mouse vision test that requires a relatively simple setup. The looming experiment was conducted in a large enclosure with a shelter in one corner and a computer monitor located on the ceiling. A CCD camera positioned next to the computer monitor served to observe mouse behavior. A mouse was placed in the enclosure for 10 minutes and allowed to acclimate to and explore the surroundings. Then, the monitor projected a program-derived looming stimulus 10 times. The mouse responded to the stimuli either by freezing or by fleeing to the hiding place. The mouse's behavior before and after the looming stimuli was recorded, and the video was analyzed using motion tracking software. The velocity of the mouse movement significantly changed after the looming stimuli. In contrast, no reaction was observed in blind mice. Our results demonstrate that the simple looming experiment is a reliable test of mouse vision.