Evidence of simian virus 40 exposure in a colony of captive baboons.
Evidence of simian virus 40 exposure in a colony of captive baboons.
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圈养狒狒群体中存在猿猴病毒 40 暴露的证据。
DOI：
10.1016/j.virol.2008.03.035
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发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Kennedy,RonaldC
中科院分区：
文献类型：
--
作者：
Westfall,LandonW;Shearer,MichaelH;Jumper,CynthiaA;White,GaryL;Papin,JamesF;Eberle,Richard;Butel,JanetS;Bright,RobertK;Kennedy,RonaldC
猿猴病毒40 （SV40）是一种多瘤病毒，非人类灵长类动物是允许宿主。狒狒（Papio spp.）是一种旧世界的猴子，用于各种研究调查；然而，狒狒之间SV40的自然感染尚未得到彻底的检查或报道。最初，我们感兴趣的是根据SV40大t抗原（Tag）抗体的存在来确定圈养狒狒群体中SV40感染的患病率。用ELISA法对142只狒狒的血清进行筛选，发现总血清阳性率为50%。142只狒狒中有5只血清抗体结合SV40 Tag的终点效价高达1280。肽结合实验表明，SV40标签的一系列表位在狒狒中具有免疫原性，并且基于表位识别，个体动物对SV40标签的体液免疫反应不同。通过对SV40标签所特有的肽表位的血清学反应，进一步检测了SV40标签的特异性，而不是其他灵长类多瘤病毒编码的大标签。另外进行血清学检测，通过Western blot评估SV40标签的反应性，以及抗体是否能够在体外中和SV40的感染性。尽管在许多狒狒中观察到具有高水平SV40中和的抗体，但病毒中和与SV40标签抗体之间缺乏相关性。利用分子诊断和SV40标签特异性实时定量PCR的进一步检查确定，一些狒狒似乎暴露于SV40。PCR产物的DNA序列分析证实在狒狒中检测到SV40标签特异性序列。
Simian virus 40 (SV40) is a polyomavirus for which non-human primates are the permissive host. The baboon (Papio spp.) is an old world monkey that is used in a variety of research investigations; however, natural infection of SV40 among baboons has not been thoroughly examined or reported. Initially, we were interested in determining the prevalence of SV40 infection among a captive colony of baboons based on the presence of antibodies to SV40 large T-antigen (Tag). An overall seroprevalence rate of >50% was found after screening sera from 142 baboons in the colony based on ELISA. Endpoint titer values for serum antibody binding to SV40 Tag reached as high as 1280 for 5 out of 142 baboons. Peptide binding assays revealed that a range of SV40 Tag epitopes are immunogenic in the baboon, and that individual animals differ in their humoral immune responses to SV40 Tag based on epitope recognition. Specificity to SV40 Tag and not some other primate polyomavirus encoded large Tag was further examined by serologic reactivity to peptide epitopes unique to SV40 Tag. Additional serology was performed to assess SV40 Tag reactivity by Western blot and whether antibodies were capable of neutralizing SV40 infectivity in vitro. Although antibodies with high levels of SV40 neutralization were observed in a number of the baboons, there was a lack of correlation between viral neutralization and antibodies to SV40 Tag. Further examination using molecular-based diagnosis and SV40 Tag specific real-time quantitative PCR determined that some of the baboons appeared to be exposed to SV40. DNA sequence analysis of the PCR products confirmed that SV40 Tag specific sequences were detected in baboons.