Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos
Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos
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DOI：
10.1038/313703a0
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发表时间：
1985-02
期刊：
影响因子：
64.8
通讯作者：
U. B. Rosenberg;A. Preiss;E. Seifert;H. Jäckle;D. C. Knipple
中科院分区：
文献类型：
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作者：
U. B. Rosenberg;A. Preiss;E. Seifert;H. Jäckle;D. C. Knipple
一个特定的信使RNA可以在与互补的多核苷酸序列的体内杂交中被功能性地灭活，这一证明表明了一种直接研究高等生物细胞中基因功能的方法1，2。这里描述的实验是为了通过互补RNA序列抑制影响果蝇胚胎命运的特定基因功能而设计的。我们使用SP6载体体外转录系统3将Krüppel（Kr）基因的部分正常非转录（无义）链转录为互补KrRNA（Krantisense RNA）。野生型果蝇胚胎，注射这种RNA，发展成为表型的Krmutant胚胎。
The demonstration that a specific messenger RNA can he functionally inactivatedin vivohy hybridization to complementary polynucleotide sequences suggests a direct approach to the study of gene function in cells of higher organisms1,2. The experiments described here were designed to inhibit, by complementary RNA sequences, a specific gene function affecting the fate of theDrosophilaembryo. We used the SP6 vectorin vitrotranscription system3to transcribe parts of the normally untranscribed (nonsense) strand of theKrüppel(Kr) gene into complementaryKrRNA (Krantisense RNA). Wild-typeDrosophilaembryos, injected with this RNA, developed into phenocopies ofKrmutant embryos.