Superinfection exclusion by P22 prophage in lysogens of Salmonella typhimurium. III. Failure of superinfecting phage DNA to enter sieA+ lysogens.
Superinfection exclusion by P22 prophage in lysogens of Salmonella typhimurium. III. Failure of superinfecting phage DNA to enter sieA+ lysogens.
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鼠伤寒沙门氏菌溶原菌中 P22 原噬菌体的重复感染排除。
DOI：
10.1016/0042-6822(74)90398-5
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发表时间：
1974
期刊：
影响因子：
3.7
通讯作者：
A. Wright
中科院分区：
文献类型：
--
作者：
M. Susskind;M. Susskind;D. Botstein;D. Botstein;A. Wright;A. Wright
Thesie 噬菌体 P22 排除系统可阻止几种沙门氏菌噬菌体的重复感染，已被证明可以阻止所有测试的重复感染噬菌体功能的表达。排除机制对于从噬菌体颗粒注射的 DNA 是特异性的，因为通过热或合子诱导的异种免疫噬菌体的生长不受 P22sieA+ 原噬菌体的存在的影响。尽管重复感染噬菌体吸附到 P22sieA+ 溶原菌，但重复感染 DNA 的注射显然是不完整的，因为该 DNA 无法被宿主 DNA 限制系统利用。虽然它没有被广泛溶解，但许多thesieA排除的DNA都有切口，这表明至少有一些DNA从噬菌体头部喷射出来。 A 排除系统阻止重复感染噬菌体 DNA 进入的假设解释了 A 系统也排除 P22 广义转导颗粒的观察结果（Ebel-Tsipis 和 Botstein，1971）。在 P22 遗传图谱上，thesieA 基因位于紧邻阻遏基因的左侧和编码参与噬菌体 DNA 注射的蛋白质的基因簇的右侧。由于 thesieA 和 mnt 基因均由受抑制的原噬菌体表达，因此它们可能受到协调控制。
ThesieA exclusion system of phage P22, which blocks superinfection by severalSalmonellaphages, has been shown to prevent expression of all superinfecting phage functions tested. The exclusion mechanism is specific for DNA injected from phage particles, since growth of heteroimmune phage by thermal or zygotic induction is unaffected by the presence of a P22sieA+prophage. Though superinfecting phage adsorb to P22sieA+lysogens, injection of superinfecting DNA is apparently incomplete, since the DNA does not become available to a host DNA restriction system. Though it is not extensively solubilized, much of thesieA-excluded DNA is nicked, indicating that at least some of it is ejected from the phage head. The hypothesis that the A exclusion system blocks entry of superinfecting phage DNA explains the observation (Ebel-Tsipis and Botstein, 1971) that the A system also excludes P22 generalized transducing particles.On the P22 genetic map, thesieA gene is located immediately to the left of themntrepressor gene and to the right of a cluster of genes coding for proteins involved in injection of phage DNA. Since both thesieA andmntgenes are expressed by the repressed prophage, it is possible that they are coordinately controlled.