JAK2-STAT3 signaling pathway is involved in rat periapical lesions induced by Enterococcus faecalis
JAK2-STAT3 signaling pathway is involved in rat periapical lesions induced by Enterococcus faecalis
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JAK2-STAT3信号通路参与粪肠球菌诱导的大鼠根尖周病变
DOI：
10.1111/odi.13169
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发表时间：
2019
期刊：
影响因子：
3.8
通讯作者：
Niu Weidong
中科院分区：
文献类型：
--
作者：
Wang Lina;Jin Haiwei;Ao Xiang;Dong Ming;Liu Shuo;Lu Ying;Niu Weidong
目的探讨JAK 2 ‐ STAT 3（Janus kinase 2/signal transducer and activator of transcription 3）在粪肠球菌（Enterococcus faecalis）根尖周病中的作用，以及脂磷壁酸（LTA）在E.材料与方法采用E.粪便被建立。HE染色证实根尖周骨吸收。用免疫组织化学染色评估JAK 2、p-JAK 2、STAT 3和p-STAT 3的表达。酶组织化学染色观察破骨细胞。LTA作用于小鼠破骨细胞前体细胞（RAW264.7细胞）; JAK 2抑制剂（AG 490）用于抑制RAW264.7细胞中的JAK 2-STAT 3通路。RAW 264. 7细胞中JAK 2-STAT 3通路和TRAP（tartrate-resistant acid phosphatase）蛋白的表达也被检测到。JAK 2 ‐ STAT 3通路中关键组分的表达随着炎症的进展而增加。LTA促进RAW264.7细胞向破骨细胞分化。结论JAK 2-STAT 3信号通路在根尖周骨吸收和破骨细胞生成过程中起重要作用。
ObjectivesThis study aimed to investigate the role of JAK2‐STAT3 (Janus kinase 2/signal transducer and activator of transcription 3) in periapical disease caused byEnterococcus faecalis, as well as the correlation between lipoteichoic acid (LTA) inE. faecalisand the activity of the JAK2‐STAT3 signaling pathway and osteoclast formation.Materials and MethodsA rat model of periapical periodontitis induced byE. faecaliswas established. Periapical bone resorption was confirmed by HE staining. The expression of JAK2, p‐JAK2, STAT3, and p‐STAT3 was assessed with immunohistochemical staining. Osteoclasts were observed through enzyme histochemical staining. LTA acted on mouse osteoclast precursor cells (RAW264.7 cells); a JAK2 inhibitor (AG490) was used to inhibit the JAK2‐STAT3 pathway in RAW264.7 cells. The expression of proteins in the JAK2‐STAT3 pathway and TRAP (tartrate resistant acid phosphatase) in RAW264.7 cells was also detected.ResultsRat periapical periodontitis was successfully established and bone resorption peaked at day 21. The expression of critical components in the JAK2‐STAT3 pathway increased with the progression of inflammation. LTA promoted the differentiation of RAW264.7 cells into osteoclasts. NFATc1 was highly expressed and was inhibited by AG490.ConclusionsJAK2‐STAT3 signaling pathway plays an important role in the process of periapical bone resorption and osteoclastogenesis.