CHROMOSOME-NUMBERS OF SAINTPAULIA-IONANTHA PLANTLETS REGENERATED FROM LEAVES CULTURED INVITRO WITH CAFFEINE AND COLCHICINE
CHROMOSOME-NUMBERS OF SAINTPAULIA-IONANTHA PLANTLETS REGENERATED FROM LEAVES CULTURED INVITRO WITH CAFFEINE AND COLCHICINE
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DOI：
10.1007/bf00038814
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发表时间：
1981-01-01
期刊：
影响因子：
1.9
通讯作者：
VAZQUEZ, AM
中科院分区：
文献类型：
--
作者：
ESPINO, FJ;VAZQUEZ, AM
部分S.在补充有不同浓度的秋水仙碱或咖啡因的培养基上培养紫罗兰叶。在不同的时间段后，将外植体转移到不含这些化学品的对照培养基中。对再生植株进行染色体计数。在所有的试验中，都有多倍体植株出现，但以秋水仙素处理的植株出现率较高。同一药剂处理的多倍体植株频率无差异。在这两种情况下，观察到高比例的细胞嵌合体植株，并给出了可能的解释。
Parts of S. ionantha leaves were cultured on a medium supplemented with different concentrations of colchicine or caffeine. The explants were transferred to a control medium without these chemicals after different periods of time. The chromosome number of the regenerated plantlets was counted. In all the experiments, polyploid plantlets were found, but the percentage of them was higher with colchicine. There were no differences in frequency of polyploid plantlets among treatments with the same chemical. In both cases a high percentage of cytochimeric plantlets was observed and possible explanations of their occurrence are given.