Effects of halothane on the transient outward K+ current in rat ventricular myocytes
Effects of halothane on the transient outward K+ current in rat ventricular myocytes
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DOI：
10.1038/sj.bjp.0703565
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发表时间：
2000-09-01
影响因子：
7.3
通讯作者：
Harrison, SM
中科院分区：
文献类型：
--
作者：
Davies, LA;Hopkins, PM;Harrison, SM
1氟烷可影响心肌组织的多种膜电流，包括L型钙电流、钠电流和多种钾电流。然而，氟烷对瞬时外向钾电流(I-to)的影响知之甚少。2用全细胞膜片结构和含EGTA的吸管溶液电压钳住大鼠单个心室肌细胞，记录I-to的钙非依赖性、4-氨基吡啶敏感成分。在+80 mV时，用300亩M Cd~(2+)或10亩M硝苯地平阻断I-Ca3，在对照条件下，I-to(脉冲结束时的峰值电流减去电流)为1.8+/-0.2nA，而1 mM氟烷使I-to减少到1.3+/-0.2nA(P
1 Halothane has been shown to affect several membrane currents in cardiac tissue including the L-type calcium current (I-Ca), sodium current and a variety of potassium currents. However, little is known about the effects of halothane on the transient outward K+ current (I-to).2 Single ventricular myocytes from rat hearts were voltage clamped using the whole cell patch configuration and an EGTA-containing pipette solution to record the Ca2+-independent, 4-aminopyridine sensitive component of I-to. 300 mu M Cd2+ or 10 mu M nifedipine was used to block I-Ca.3 At +80 mV, I-to (peak current minus current at the end of the pulse) was 1.8+/-0.2 nA under control conditions which was reduced to 1.3+/-0.2 nA by 1 mM halothane (P