HUMAN BASIC FIBROBLAST GROWTH-FACTOR GENE ENCODES 4 POLYPEPTIDES - 3 INITIATE TRANSLATION FROM NON-AUG CODONS
HUMAN BASIC FIBROBLAST GROWTH-FACTOR GENE ENCODES 4 POLYPEPTIDES - 3 INITIATE TRANSLATION FROM NON-AUG CODONS
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DOI：
10.1073/pnas.86.11.3978
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发表时间：
1989-06-01
影响因子：
11.1
通讯作者：
SOMMER, A
中科院分区：
文献类型：
--
作者：
FLORKIEWICZ, RZ;SOMMER, A
人碱性成纤维细胞生长因子（bFGF）是一种存在于多种中胚层和神经外胚层来源的组织中的血管生成多肽丝裂原。bFGF cDNA和基因组克隆预测一个17.8 kDa（155个氨基酸）的基因产物的基础上存在一个单一的假定翻译起始ATG密码子。然而，从人胎盘中分离的bFGF蛋白质含有两个额外的氨基酸NH 2-末端的预测起始蛋氨酸。我们在这里报告，人类细胞系SK-HEP-1共表达四种分子形式（17.8，22.5，23.1和24.2 kDa）的bFGF。17.8-kDa bFGF蛋白质在先前预测的甲硫氨酸（AUG）密码子处起始，而22.5-、23.1-和24.2-kDa蛋白质起始不寻常的非AUG密码子。较高分子量的形式是18-kDa bFGF的共线NH 2-末端延伸。
Human basic fibroblast growth factor (bFGF) is an angiogenic polypeptide mitogen present in a wide variety of mesoderm- and neuroectoderm-derived tissues. bFGF cDNA and genomic clones predict a 17.8-kDa (155-amino acid) gene product based on the presence of a single putative translational initiator ATG codon. However, a bFGF protein isolated from human placenta contains two additional amino acids NH2-terminal to the predicted initiator methionine. We report here that the human cell line SK-HEP-1 coexpresses four molecular forms (17.8, 22.5, 23.1, and 24.2 kDa) of bFGF. The 17.8-kDa bFGF protein is translationally initiated at the previously predicted methionine (AUG) codon, whereas the 22.5-, 23.1-, and 24.2-kDa proteins initiate a unusual non-AUG codons. The higher molecular weight forms are colinear NH2-terminal extensions of the 18-kDa bFGF.