Metagenomic analysis of viruses in reclaimed water
Metagenomic analysis of viruses in reclaimed water
复制标题
DOI：
10.1111/j.1462-2920.2009.01964.x
复制
发表时间：
2009-11-01
影响因子：
5.1
通讯作者：
Breitbart, Mya
中科院分区：
文献类型：
--
作者：
Rosario, Karyna;Nilsson, Christina;Breitbart, Mya
再生水利用是可持续水资源管理的一个重要组成部分。然而，有人担心病原体通过这种替代水源的传播。本研究描述了再生水中发现的病毒群落，并将其与饮用水中的病毒进行了比较。再生水含有比饮用水多1000倍的病毒样颗粒，每毫升约有10（8）个VLP。宏基因组分析显示，再生水和饮用水中的大多数病毒都是新的。噬菌体占主导地位的DNA病毒社区在再生水和饮用水，但再生水有一个独特的噬菌体社区的基础上，噬菌体家族分布和每个家庭内的主机代表。与植物病原体和无脊椎动物小核糖核酸病毒相似的真核病毒主导RNA宏基因组文库。在再生水病毒宏基因组中未检测到已建立的人类病原体，其中含有丰富的与植物、动物和昆虫病毒相关的新型单链DNA和RNA病毒。因此，再生水可能在高稳定性病毒的传播中发挥作用。关于再生水中存在但饮用水中不存在的病毒的信息可用于确定新的水质生物指标。未来的研究将需要调查这些病毒的感染性和宿主范围，以评估再生水使用对人类和生态系统健康的影响。
Reclaimed water use is an important component of sustainable water resource management. However, there are concerns regarding pathogen transport through this alternative water supply. This study characterized the viral community found in reclaimed water and compared it with viruses in potable water. Reclaimed water contained 1000-fold more virus-like particles than potable water, having approximately 10(8) VLPs per millilitre. Metagenomic analyses revealed that most of the viruses in both reclaimed and potable water were novel. Bacteriophages dominated the DNA viral community in both reclaimed and potable water, but reclaimed water had a distinct phage community based on phage family distributions and host representation within each family. Eukaryotic viruses similar to plant pathogens and invertebrate picornaviruses dominated RNA metagenomic libraries. Established human pathogens were not detected in reclaimed water viral metagenomes, which contained a wealth of novel single- stranded DNA and RNA viruses related to plant, animal and insect viruses. Therefore, reclaimed water may play a role in the dissemination of highly stable viruses. Information regarding viruses present in reclaimed water but not in potable water can be used to identify new bioindicators of water quality. Future studies will need to investigate the infectivity and host range of these viruses to evaluate the impacts of reclaimed water use on human and ecosystem health.