Aberrant expressions of stem cell factor/c-KIT in rat testis with varicocele
Aberrant expressions of stem cell factor/c-KIT in rat testis with varicocele
复制标题
精索静脉曲张大鼠睾丸中干细胞因子/c-KIT的异常表达
DOI：
10.1016/j.jfma.2016.09.001
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发表时间：
2017
影响因子：
3.2
通讯作者：
Dong Lina
中科院分区：
文献类型：
--
作者：
Qin Qin;Liu Jianrong;Ma Yuehong;Wang Yimin;Zhang Fang;Gao Songdan;Dong Lina
背景/目的精索静脉曲张（VC）被世界卫生组织认为是男性不育的主要原因。研究表明维生素C可影响精子发生，进而导致男性不育。但VC影响精子发生的确切机制尚不清楚。干细胞因子（SCF）和c-KIT受体是睾丸精子发生过程中的重要分子。本研究旨在探讨SCF/c-KIT信号通路是否参与VC对精子发生的病理生理过程。HE染色观察睾丸曲细精管形态，酶联免疫吸附试验和Western blot检测SCF表达，Western blot和免疫荧光检测c-KIT表达。结论VC对生精上皮精子数有影响，VC睾丸中SCF/c-KIT系统表达异常，可能参与了VC所致男性不育的发生。
Background/PurposeVaricocele (VC) is considered by the World Health Organization as the main cause of male infertility. Studies have shown that VC can affect spermatogenesis and then result in male infertility. But the exact mechanism by which VC affects spermatogenesis is still unclear. Stem cell factor (SCF) and c-KIT receptor are crucial molecules during spermatogenesis in testis. This study aims to investigate whether SCF/c-KIT signaling is involved in the pathophysiology of VC on spermatogenesis.MethodsRat models of VC were built (n= 13), and sham-operated rats were used as controls (n= 8). The seminiferous tubules of the testis were observed with hematoxylin and eosin staining, expression of SCF was analyzed via enzyme-linked immunosorbent assay and Western blot, and expression of c-KIT was assessed with Western blot and immunofluorescence.ResultsCompared with controls, the seminiferous epithelium was disorganized and had significantly fewer cells in the testes of rats with VC. Expression of SCF increased in testes of VC rats, while expression of c-KIT was decreased.ConclusionThese results suggest that sperm counts in seminiferous epithelium are affected by VC, and the SCF/c-KIT system is aberrantly expressed in VC testis, which could be involved in male infertility caused by VC.