A rat model of studying tissue-type plasminogen activator thrombolysis in ischemic stroke with diabetes.
A rat model of studying tissue-type plasminogen activator thrombolysis in ischemic stroke with diabetes.
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DOI：
10.1161/strokeaha.111.635250
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发表时间：
2012-02
期刊：
影响因子：
8.3
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Fan X;Qiu J;Yu Z;Dai H;Singhal AB;Lo EH;Wang X
中风后高血糖和糖尿病与较低的溶栓效果和增加的缺血后脑出血风险相关。我们的目的是开发一种模拟临床情况的糖尿病卒中溶栓啮齿动物模型。雄性 6 周 I 型糖尿病大鼠（14 周龄）进行栓塞性局灶性中风，并在 1.5 小时时接受 tPA 治疗。测量再灌注和 24 小时神经学结果并与非糖尿病对照大鼠进行比较。糖尿病大鼠表现出对溶栓再灌注的抵抗、更大的梗塞体积和增加的脑出血。该动物模型将与未来研究病理生理学机制和开发新的治疗方法以增强 tPA 溶栓治疗糖尿病或中风后高血糖患者的疗效相关。
Post-stroke hyperglycemia and diabetes mellitus are associated with lower thrombolytic efficacy and an increased risk of post-ischemic cerebral hemorrhage. We aimed to develop a rodent model of thrombolysis in diabetic stroke that mimics the clinical situation. Male 6 weeks type I diabetic rats (14 weeks old) rats were subjected to embolic focal stroke and treated with tPA at 1.5 hours. Reperfusion and 24-hour neurological outcomes were measured and compared to non-diabetic control rats. Diabetic rats exhibited resistance to thrombolytic reperfusion, larger infarction volumes, and increased intracerebral hemorrhage. This animal model would be relevant to future studies investigating pathophysiological mechanisms and in developing new therapeutic approaches to enhance the efficacy of tPA thombolysis in stroke patients with diabetes or post-stroke hyperglycemia.