Teichoic acids are temporal and spatial regulators of peptidoglycan cross-linking in Staphylococcus aureus
Teichoic acids are temporal and spatial regulators of peptidoglycan cross-linking in Staphylococcus aureus
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DOI：
10.1073/pnas.1004304107
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发表时间：
2010-11-02
影响因子：
11.1
通讯作者：
Filipe, Sergio R.
中科院分区：
文献类型：
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作者：
Atilano, Magda L.;Pereira, Pedro M.;Filipe, Sergio R.
金黄色葡萄球菌的细胞壁以其肽聚糖(PGN)内极高的交联度为特征。青霉素结合蛋白4(PBP4)是合成这种高度交联的肽聚糖所必需的。我们发现，壁磷壁酸是附着在肽聚糖上的糖聚合物，对革兰氏阳性细菌的毒力至关重要，它作为PGN代谢的时空调节因子，通过调节PBP4的定位来控制交联物的水平。PBP4通常定位于分裂隔膜，但在没有壁磷壁酸合成的情况下，它会分散在整个细胞膜上，无法正常发挥功能。因此，Tago Null突变体的肽聚糖在壁磷壁酸的生物合成中受到损害，其交联度降低，这使得它更容易受到溶菌酶的作用，溶菌酶是不同宿主生物产生的一种酶，作为抵御细菌感染的初始防御。
The cell wall of Staphylococcus aureus is characterized by an extremely high degree of cross-linking within its peptidoglycan (PGN). Penicillin-binding protein 4 (PBP4) is required for the synthesis of this highly cross-linked peptidoglycan. We found that wall teichoic acids, glycopolymers attached to the peptidoglycan and important for virulence in Gram-positive bacteria, act as temporal and spatial regulators of PGN metabolism, controlling the level of cross-linking by regulating PBP4 localization. PBP4 normally localizes at the division septum, but in the absence of wall teichoic acids synthesis, it becomes dispersed throughout the entire cell membrane and is unable to function normally. As a consequence, the peptidoglycan of TagO null mutants, impaired in wall teichoic acid biosynthesis, has a decreased degree of cross-linking, which renders it more susceptible to the action of lysozyme, an enzyme produced by different host organisms as an initial defense against bacterial infection.