Extraction of Nucleic Acids from Bone

Extraction of Nucleic Acids from Bone
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DOI:
10.1007/978-1-61779-415-5_17
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发表时间:
2012-01-01
期刊:
BONE RESEARCH PROTOCOLS, SECOND EDITION
影响因子:
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通讯作者:
Mann, Val
Mann, Val
中科院分区:
其他
文献类型:
--
作者:
Hughes, Alun;Stewart, Tracy L.;Mann, Val

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在这里,我们提出了从全骨组织和培养骨细胞样品中提取 DNA 和 RNA 的方法,并描述了提取核酸的定量和定性测量方法。所描述的这些方案提供了适合下游应用(例如定量 PCR 和微阵列)的高质量核酸。
Here, we present methods for extracting DNA and RNA from samples of whole bone tissue and culture bone cells and describe methods quantitative and qualitative measurement of the extracted nucleic acids. These protocols described provide high-quality nucleic acids suitable for downstream applications such as quantitative PCR and microarrays.