Establishment of cell lines derived from the rat suprachiasmatic nucleus
Establishment of cell lines derived from the rat suprachiasmatic nucleus
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DOI：
10.1016/j.bbrc.2007.02.009
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发表时间：
2007-04-06
影响因子：
3.1
通讯作者：
Tei, Hajime
中科院分区：
文献类型：
--
作者：
Kawaguchi, Soshi;Shinozaki, Atsuki;Tei, Hajime
哺乳动物的生理和行为昼夜节律是由位于下丘脑视交叉上核(SCN)的中央昼夜节律编排的。光输入牵涉到中央时钟的相位，而许多外周时钟受到来自SCN的神经或激素输出的调节。我们建立了来源于大鼠胚胎SCN的细胞系，以检测中央时钟的分子网络。建立的细胞系表现出时钟基因稳定的昼夜节律表达。这种昼夜振荡被forsklin突然相移，并被siBMal1消除。这些结果与SCN的体内研究是一致的。(C)2007 Elsevier Inc.保留所有权利。
Physiological and behavioral circadian rhythms in mammals are orchestrated by a central circadian clock located in the suprachiasmatic nucleus (SCN) of the hypothalamus. Photic input entrains the phase of the central clock, and many peripheral clocks are regulated by neural or hormonal output from the SCN. We established cell lines derived from the rat embryonic SCN to examine the molecular network of the central clock. An established cell line exhibited the stable circadian expression of clock genes. The circadian oscillation was abruptly phase-shifted by forskolin, and abolished by siBmal1. These results are compatible with in vivo studies of the SCN. (c) 2007 Elsevier Inc. All rights reserved.