Mechanisms and enzymes involved in SARS coronavirus genome expression
Mechanisms and enzymes involved in SARS coronavirus genome expression
复制标题
DOI：
10.1099/vir.0.19424-0
复制
发表时间：
2003-09-01
影响因子：
3.8
通讯作者：
Ziebuhr, J
中科院分区：
文献类型：
--
作者：
Thiel, V;Ivanov, KA;Ziebuhr, J
一种新型冠状病毒是目前严重急性呼吸综合征（SARS）流行的病原体。冠状病毒是非常大的RNA病毒，并采用复杂的调控机制来表达其基因组。在这里，我们确定了SARS冠状病毒（SARS-CoV）的序列，分离法兰克福1，并表征了病毒基因组表达中涉及的关键RNA元件和蛋白质功能。重要的调控机制，如（不连续）合成的8个亚基因组mRNA，核糖体移码和翻译后蛋白水解加工，解决。三个SARS冠状病毒酶，解旋酶和两个半胱氨酸蛋白酶，这是已知的关键参与复制，转录和/或翻译后多蛋白加工的活动，其特征在于。SARS冠状病毒关键复制酶的重组形式的可用性为高通量筛选方法铺平了道路，以确定化合物库中的候选抑制剂。
A novel coronavirus is the causative agent of the current epidemic of severe acute respiratory syndrome (SARS). Coronaviruses are exceptionally large RNA viruses and employ complex regulatory mechanisms to express their genomes. Here, we determined the sequence of SARS coronavinus (SARS-CoV), isolate Frankfurt 1, and characterized key RNA elements and protein functions involved in viral genome expression. Important regulatory mechanisms, such as the (discontinuous) synthesis of eight subgenomic mRNAs, ribosomal frameshifting and post-translational proteolytic processing, were addressed. Activities of three SARS coronavirus enzymes, the helicase and two cysteine proteinases, which are known to be critically involved in replication, transcription and/or post-translational polyprotein processing, were characterized. The availability of recombinant forms of key replicative enzymes of SARS coronavirus should pave the way for high-throughput screening approaches to identify candidate inhibitors in compound libraries.