Comparison of the ability of viral protein-expressing plasmid DNAs to protect against influenza
Comparison of the ability of viral protein-expressing plasmid DNAs to protect against influenza
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DOI：
10.1016/s0264-410x(98)00043-7
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发表时间：
1998-10-01
期刊：
影响因子：
5.5
通讯作者：
Tamura, S
中科院分区：
文献类型：
--
作者：
Chen, Z;Sahashi, Y;Tamura, S
在BALB/c小鼠中比较编码来自A/PR/8/34（H1N1）病毒的各种流感病毒蛋白的质粒DNA保护免受流感的能力。质粒DNA在鸡β-肌动蛋白表达载体（pCAGGS）中编码血凝素（HA）、神经氨酸酶（NA）、基质蛋白（M1）、核蛋白（NP）或非结构蛋白（NS 1）。通过颗粒介导的DNA转移至表皮（基因枪），每种DNA以每只小鼠1 μ g的剂量接种两次，间隔3周。第二次免疫后7天，用同源病毒攻击小鼠，并通过降低的肺病毒滴度和增加的存活率来评估每种DNA保护小鼠免受流感的能力。小鼠，给予HA-或NA-表达DNA，诱导高水平的特异性抗体反应，并保护好对攻击病毒。另一方面，给予M1-、NP-或NS 1-DNA的小鼠未能提供保护，尽管M1-和NP-DNA确实诱导了可检测的抗体应答。这些结果表明，在这里使用的各种表达病毒蛋白的DNA中，表面糖蛋白的HA和NA表达DNA对流感的保护性最强。(C)1998 Elsevier Science Ltd保留所有权利。
The ability of plasmid DNA encoding various influenza viral proteins from the A/PR/8/34 (H1N1) virus to protect against influenza was compared in BALB/c mice. The plasmid DNA encoded hemagglutinin (HA), neuraminidase (NA), matrix protein (M1), nucleoprotein (NP) or nonstructural protein (NS1) in a chicken beta-actin-based expression vector (pCAGGS). Each DNA was inoculated twice 3 weeks apart at a dose of 1 mu g per mouse by particle-mediated DNA transfer to the epidermis (gene gun). Seven days after a second immunization, mice were challenged with the homologous virus and the ability, of each DNA to protect mice from influenza was evaluated by decreased lung virus titers and increased survival. Mice, given HA- or NA-expressing DNA, induced a high level of specific antibody response and protected well against the challenge virus. On the other hand, mice given M1-, NP-, or NS1-DNA failed to provide protection, although M1- and NP-DNAs did induce detectable antibody responses. These results indicate that both HA- and NA-expressing DNAs for the surface glycoproteins are most protective against influenza from among the various viral protein-expressing DNAs used here. (C) 1998 Elsevier Science Ltd All rights reserved.